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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Rhizobium etli (strain CIAT 652)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19UniRule annotation1
Active sitei147UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 115ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL491916:G1GBZ-963-MONOMER
UniPathwayiUPA00056; UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:RHECIAT_CH0000963
OrganismiRhizobium etli (strain CIAT 652)
Taxonomic identifieri491916 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008817 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000921081 – 2974-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST297

Proteomic databases

PRIDEiB3PRG5

Interactioni

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0000963

Structurei

3D structure databases

ProteinModelPortaliB3PRG5
SMRiB3PRG5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTR Bacteria
COG1947 LUCA
HOGENOMiHOG000019600
KOiK00919
OMAiRWPSPAK
OrthoDBiPOG091H00XD

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PANTHERiPTHR43527:SF2 PTHR43527:SF2, 1 hit
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

B3PRG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEAGLAEAF GVTEEARAKI NLALHVTGQR PDGYHLLEML VTFADCGDRL
60 70 80 90 100
GFLPAQADAF TLSGPFGAML AGDGNNLVLR ARDLLREQVG ALAFPVHIHL
110 120 130 140 150
QKNLPIASGI GGGSADAAAT LRGLMRLWGM DLPAETLASL ALTLGADVPM
160 170 180 190 200
CFESRPLIAR GIGEKIEAVP DLPAFAMVLA NPLKGVSTPE VFRRLAAKNN
210 220 230 240 250
PALHLARSLS ATSDWLTAIG SARNDLEPPA CELVPEIAMI SQMLQAQGAL
260 270 280 290
ITRMSGSGAT CFGIFSTMTA AQEAAAALHG ERPDWYVQAT ETVSGGM
Length:297
Mass (Da):31,126
Last modified:September 2, 2008 - v1
Checksum:iB1907A9C9EAEE2D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA Translation: ACE89948.1
RefSeqiWP_012482861.1, NC_010994.1

Genome annotation databases

EnsemblBacteriaiACE89948; ACE89948; RHECIAT_CH0000963
KEGGirec:RHECIAT_CH0000963

Similar proteinsi

Entry informationi

Entry nameiISPE_RHIE6
AccessioniPrimary (citable) accession number: B3PRG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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