B3PRD6 (LPXK_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RHECIAT_CH0000934

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000123731
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3PRD6 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 042B1133C459742A	FASTA	347	37,536
	10 20 30 40 50 60 MISEAPPFWW RKADWRAWML APLSFVYGRV AGHRMAHARR ASVSIPVICV GNFTVGGAGK 70 80 90 100 110 120 TPTALTIARA AKAKGLKPGF LSRGYGGSLD VTTVVDPHHH RAVAVGDEPL LLAQEALTVI 130 140 150 160 170 180 SRRRVDGAHR LVAEGADLII MDDGFQSARL AIDYALLVID ATRGLGNGHI VPAGPVRAPI 190 200 210 220 230 240 GQQLRSATAL LKVGGGQAAD RIVRMAARAA KPYFTASLKV RGDDRLTGIR VLAFAGIADP 250 260 270 280 290 300 AKFFRTVESR GAEITVAKTF GDHEHLSEDE IGDILTTAER QDLLIVTTSK DFVRLSGHHG 310 320 330 340 KAQELAQQCR VIEVDMVFDD HLAPGLIIDR AIVACRERRL REMKTKS

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIAT 652.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE89919.1.
RefSeq	YP_001977097.1. NC_010994.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3PRD6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6399021.
GenomeReviews	Gene locus lpxK in contig CP001074_GR.
KEGG	rec:RHECIAT_CH0000934.
PATRIC	23095309. VBIRhiEtl120572_0914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHIE6

Accession

Primary (citable) accession number: B3PRD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program