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Protein

Enolase

Gene

eno

Organism
Mycoplasma arthritidis (strain 158L3-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgm)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei161SubstrateUniRule annotation1
Binding sitei170SubstrateUniRule annotation1
Active sitei211Proton donorUniRule annotation1
Metal bindingi252MagnesiumUniRule annotation1
Metal bindingi314MagnesiumUniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Metal bindingi341MagnesiumUniRule annotation1
Binding sitei341SubstrateUniRule annotation1
Active sitei366Proton acceptorUniRule annotation1
Binding sitei366Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei417SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:MARTH_orf859
OrganismiMycoplasma arthritidis (strain 158L3-1)
Taxonomic identifieri243272 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000008812 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001158861 – 456EnolaseAdd BLAST456

Proteomic databases

PRIDEiB3PNH6.

Interactioni

Protein-protein interaction databases

STRINGi243272.MARTH_orf859.

Structurei

3D structure databases

ProteinModelPortaliB3PNH6.
SMRiB3PNH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni393 – 396Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiPOG091H02DK.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.

Sequencei

Sequence statusi: Complete.

B3PNH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIVSINAY EVLDSRGNPT VKVELTTEKA YAEALVPSGA STGSKEALEL
60 70 80 90 100
RDKGTKYEKN WFGGKGVQTA VDNVNEIIFP ALKGKDVTKQ FEIDKLMIEL
110 120 130 140 150
DGTETKSKLG ANAILAVSLA VAKAAANEAN VPLYAYLAKL DNRQAYKLPV
160 170 180 190 200
PMLNVINGGE HASNTIDFQE FMIMPLGAKT FKEAMQIANF VFHTLAKLLK
210 220 230 240 250
EAGHGTQVGD EGGFAPNLHT HEETLDFLVN AIKKAGYNPA TSGDNAVAIC
260 270 280 290 300
LDTASSELYC SESKTYTFKK FKKALDEKRP GFEKYASMKY KFTSDEYVEY
310 320 330 340 350
YGNLIAKYPI ISIEDSHDEN DWEGFRKMKK LYGNRVQLVG DDLIVTNPKY
360 370 380 390 400
IKMAIEKDAI NASLIKINQI GSLWETIEAI KMTQAANMVP VISHRSGETE
410 420 430 440 450
DTFIADLAVA FNTGEIKTGS LSRTDRIAKY NRLLKIEQEL GNKAKYEGRA

SFSNLK
Length:456
Mass (Da):50,403
Last modified:September 2, 2008 - v1
Checksum:iBCCD3A9AAB41FD48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001047 Genomic DNA. Translation: ACF07578.1.
RefSeqiWP_012498535.1. NC_011025.1.

Genome annotation databases

EnsemblBacteriaiACF07578; ACF07578; MARTH_orf859.
KEGGimat:MARTH_orf859.
PATRICi20004409. VBIMycArt31993_0624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001047 Genomic DNA. Translation: ACF07578.1.
RefSeqiWP_012498535.1. NC_011025.1.

3D structure databases

ProteinModelPortaliB3PNH6.
SMRiB3PNH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243272.MARTH_orf859.

Proteomic databases

PRIDEiB3PNH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF07578; ACF07578; MARTH_orf859.
KEGGimat:MARTH_orf859.
PATRICi20004409. VBIMycArt31993_0624.

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiPOG091H02DK.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_MYCA5
AccessioniPrimary (citable) accession number: B3PNH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: April 12, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.