B3PN19 (SYE_MYCA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MARTH_orf641

Organism

Mycoplasma arthritidis (strain 158L3-1) [Complete proteome] [HAMAP]

Taxonomic identifier

243272 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma arthritidis

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_1000090090
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	253 – 257	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	256	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PN19 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 151CEA98284D39AE	FASTA	464	53,707
	10 20 30 40 50 60 MNKTIRTRYA PSPTGYLHIG GARTALFCYL FAKHSNGDFI FRLEDTDVER NVEGGEASQL 70 80 90 100 110 120 NNLAWLGIVP DESPLKPNPK YGKYRQSEKL AIYQAYIDEL IKQGLAYKAY DSSEELAKQH 130 140 150 160 170 180 EEQEKAGVAS FRYDPTWLKL SESEIKRRDE AKEYSIRLKL PKNKNYSWDD LVRGPISVNS 190 200 210 220 230 240 DDIGDFVIMK SDGYPTYNFA VVVDDHQMDI THVLRGEEHI TNTPKQLAIY EAFGWDNPVF 250 260 270 280 290 300 GHLTIITNME GKKLSKRDKS LKQFIEDYKN EGYCPEAIFN FLALLGWTSG DKTEIMSHDE 310 320 330 340 350 360 LIKKFDYNRL SKSPSKFDIV KMEWFSKQYM KKLPNEVIIE KINSPKDAQW NNLFVETYKQ 370 380 390 400 410 420 QAATISEIKE NLKIYLFPKE KLELQIDNDL VVKTFFAHLK AKDFTIENIQ AAIDETKNAL 430 440 450 460 NVKGKDLFMP IRITATYEEH GPELAKAIYL FGKDLVYKRL TKWS

References

[1]	"Genome of Mycoplasma arthritidis." Dybvig K., Zuhua C., Lao P., Jordan D.S., French C.T., Tu A.H., Loraine A.E. Infect. Immun. 76:4000-4008(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 158L3-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001047 Genomic DNA. Translation: ACF07421.1.
RefSeq	YP_002000125.1. NC_011025.1.
3D structure databases
ProteinModelPortal	B3PN19.
ModBase	Search...
Protein-protein interaction databases
STRING	243272.MARTH_orf641.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF07421; ACF07421; MARTH_orf641.
GeneID	6418126.
KEGG	mat:MARTH_orf641.
PATRIC	20004069. VBIMycArt31993_0460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252720.
KO	K01885.
OMA	AFRCFCT.
OrthoDB	EOG6DRPF7.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MART243272:GHIZ-483-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_bac/mito. IPR000924. Glu/Gln-tRNA-synth. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. SSF48163. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCA5

Accession

Primary (citable) accession number: B3PN19

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	November 13, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program