B3PL16 (SYT_CELJU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	CJA_2530

Organism

Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000098554
Regions
	Region	243 – 534	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	511	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PL16 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 8CA68EA105D8B374	FASTA	642	73,298
	10 20 30 40 50 60 MPVITLPDGS QRSFDKPVSV YEVAASIGPG LAKATLGGRV NGQRVDAHDL ISEDANLVIF 70 80 90 100 110 120 TPKDDDGLEI IRHSCAHLLG HAIKQLYPDV KMAIGPTIDN GFYYDVDLEH KLTDEDIAAL 130 140 150 160 170 180 EARMLELAKT EYDVVKKKVS WQEAHAVFAA RGESYKLEIL ERDIPQSDQP GLYHHEEYID 190 200 210 220 230 240 MCRGPHVPNM RFCLNFKLMR VSGAYWRGDS NNKMLQRIYG TAFADAKQLK AYLNLLEEAA 250 260 270 280 290 300 KRDHRKLAKK FDLFHLQDEA PGMVFWHPKG WTLWQQIEQY MRAKQNDWGY QEVRTPLIMD 310 320 330 340 350 360 RVLWEKSGHW ENYRENMFTT ESEKRDFAVK PMNCPGHVQI FNHGLRSHKD LPLRLAEFGS 370 380 390 400 410 420 CHRNEASGAL HGIMRVRGFV QDDAHIFCTE DQVVAEVAAF NQMLKSVYDD FGFTDIAVKL 430 440 450 460 470 480 SLRPEKYAGS LETWNKAEEG LRTALRAAGL EWEELPGEGA FYGPKIEYQI KDALGRSWQC 490 500 510 520 530 540 GTIQLDYVLP ERLDAEYVAD DNVRKRPVML HRAILGSFER FLGILIEHYE GSFPVWLSPV 550 560 570 580 590 600 QVVVANITDS QAEYVKRVEK ALREQGIRVI ADLRNEKIGF KIREHTIQRV PYLLVVGDKE 610 620 630 640 VENQSLAVRT RDGKDLGVMP LDAFIAHLTA DIARRGRVFS AE

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ueda107.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000934 Genomic DNA. Translation: ACE83885.1.
RefSeq	YP_001982990.1. NC_010995.1.
3D structure databases
ProteinModelPortal	B3PL16.
ModBase	Search...
Protein-protein interaction databases
STRING	B3PL16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6414923.
GenomeReviews	Gene locus CJA_2530 in contig CP000934_GR.
KEGG	cja:CJA_2530.
PATRIC	21328438. VBICelJap122165_2487.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	MIIRNIL.
Enzyme and pathway databases
BioCyc	CJAP155077:CJA_2530-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_CELJU

Accession

Primary (citable) accession number: B3PL16

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program