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Protein

NAD kinase

Gene

nadK

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771Proton acceptorUniRule annotation
Binding sitei162 – 1621NADUniRule annotation
Binding sitei179 – 1791NADUniRule annotation
Binding sitei181 – 1811NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 782NADUniRule annotation
Nucleotide bindingi151 – 1522NADUniRule annotation
Nucleotide bindingi192 – 1976NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-2166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:CJA_2174
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
ProteomesiUP000001036: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300NAD kinasePRO_1000120838Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_2174.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B3PJ64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHFSTIGLI GHLNNERAVY SIERLIRFLQ QRGKDFVLEV ETAARISDIA
60 70 80 90 100
LTQAARQIMD MDALGQICDL VIVVGGDGSL LSGARALAKY QVPLLGVNRG
110 120 130 140 150
RLGFLTDITP EQIEQKMAEV LTGQFASEKR FLLDMEVRRD GQVIALADAL
160 170 180 190 200
NDVVLHTGQF IHMLEFEIHV DGSFVTSQRS DGLIVSTPTG STAYSLSGGG
210 220 230 240 250
PILHPKLDAI VIVPMNPHTL SSRPIVVSGD SEILLMVGEH NRALPMVTCD
260 270 280 290 300
GHSHAEVQTG DEIIIRKKPQ LLELLHPLDY NFYERCRSKL GWGGHLLKPE
Length:300
Mass (Da):32,991
Last modified:September 2, 2008 - v1
Checksum:i47507CFA9FC0713A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE86035.1.
RefSeqiWP_012487776.1. NC_010995.1.
YP_001982639.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE86035; ACE86035; CJA_2174.
GeneIDi6416582.
KEGGicja:CJA_2174.
PATRICi21327734. VBICelJap122165_2138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE86035.1.
RefSeqiWP_012487776.1. NC_010995.1.
YP_001982639.1. NC_010995.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498211.CJA_2174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE86035; ACE86035; CJA_2174.
GeneIDi6416582.
KEGGicja:CJA_2174.
PATRICi21327734. VBICelJap122165_2138.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-2166-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.

Entry informationi

Entry nameiNADK_CELJU
AccessioniPrimary (citable) accession number: B3PJ64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: February 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.