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B3PIB4 (SYL_CELJU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CJA_0454
OrganismCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]
Taxonomic identifier498211 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091301

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3PIB4 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: EBC31552266D6203

FASTA86497,610
        10         20         30         40         50         60 
MQEQYNPAEV EAQAQQYWEE HQSFNVIEDP GKEKFYCLAM FPYPSGKLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARYQRMQG KNVLHPMGWD AFGLPAENAA LKHNTAPAKW TYSNTDHMRN QLKQLGFGFD 

       130        140        150        160        170        180 
WSRELTTCKP EYYQWEQWFF TRLYEKGLVY KKMSTVNWDP IDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVERKEIPQ WFIKITDYAE ELLNDLDKLP NWPEQVKTMQ RNWIGKSRGL EMRFDLQSPV 

       250        260        270        280        290        300 
GEFTSFDIYT TRPDTLMGVT YVSLAAEHPI AKYLADSNPA LAQFIADCKV QSVAEADMAT 

       310        320        330        340        350        360 
MEKKGMDTGI KALHPITGEP VAVWVANYVL MDYGSGAVMA VPAHDQRDYE FAQKYHLGIT 

       370        380        390        400        410        420 
QVIAPQNGET IDLSQAAFTD KGVLVNSGEY DGLDFNAAFD AIASTLEMAN KGRVKTNYRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGAPIPMFNL PEGGEIPVPA HKLPILLPEE VVMNGVQSPI KADPEWKKDE 

       490        500        510        520        530        540 
LDGQYVERET DTFDTFMESS WYYARYTCPN FTDGMINKAA ADYWLPVDQY VGGIEHAILH 

       550        560        570        580        590        600 
LLYSRFFHKL MRDEGLVSGD EPFERLLCQG MVNAESFFIK SEGKENWIEP ENVVIERDDK 

       610        620        630        640        650        660 
GRFIAAKHKI TGDAVEFGGV IKMSKSKANG VDPESVINQY GADTVRLFTM FAAPPEQSLE 

       670        680        690        700        710        720 
WSDSGVEGAS RFLRRLWKAV EGHINAGTPG KLDAASLPQQ QKDLRRKTHE TIQKVSDDYG 

       730        740        750        760        770        780 
RRQTFNTAIA AVMELLNETS KLSDRANPQG LAVEREALEA AILLLAPIVP HITQALWIEL 

       790        800        810        820        830        840 
GNSGIPLNQP WPTLDESALV RSTIEVVVQV NGKLRGKIDA AVDAPKELLE QIAVQQENVQ 

       850        860 
KFLEGVTVRK VIVVPNKLVN IVAN 

« Hide

References

[1]"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ueda107.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000934 Genomic DNA. Translation: ACE83294.1.
RefSeqYP_001980977.1. NC_010995.1.

3D structure databases

ProteinModelPortalB3PIB4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498211.CJA_0454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE83294; ACE83294; CJA_0454.
GeneID6414491.
KEGGcja:CJA_0454.
PATRIC21324289. VBICelJap122165_0464.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCJAP498211:GHIT-454-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CELJU
AccessionPrimary (citable) accession number: B3PIB4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries