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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-454-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CJA_0454
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
ProteomesiUP000001036 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Leucine--tRNA ligasePRO_1000091301Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_0454.

Structurei

3D structure databases

ProteinModelPortaliB3PIB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3PIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPAEV EAQAQQYWEE HQSFNVIEDP GKEKFYCLAM FPYPSGKLHM
60 70 80 90 100
GHVRNYTITD VIARYQRMQG KNVLHPMGWD AFGLPAENAA LKHNTAPAKW
110 120 130 140 150
TYSNTDHMRN QLKQLGFGFD WSRELTTCKP EYYQWEQWFF TRLYEKGLVY
160 170 180 190 200
KKMSTVNWDP IDQTVLANEQ VIDGRGWRSG ALVERKEIPQ WFIKITDYAE
210 220 230 240 250
ELLNDLDKLP NWPEQVKTMQ RNWIGKSRGL EMRFDLQSPV GEFTSFDIYT
260 270 280 290 300
TRPDTLMGVT YVSLAAEHPI AKYLADSNPA LAQFIADCKV QSVAEADMAT
310 320 330 340 350
MEKKGMDTGI KALHPITGEP VAVWVANYVL MDYGSGAVMA VPAHDQRDYE
360 370 380 390 400
FAQKYHLGIT QVIAPQNGET IDLSQAAFTD KGVLVNSGEY DGLDFNAAFD
410 420 430 440 450
AIASTLEMAN KGRVKTNYRL RDWGVSRQRY WGAPIPMFNL PEGGEIPVPA
460 470 480 490 500
HKLPILLPEE VVMNGVQSPI KADPEWKKDE LDGQYVERET DTFDTFMESS
510 520 530 540 550
WYYARYTCPN FTDGMINKAA ADYWLPVDQY VGGIEHAILH LLYSRFFHKL
560 570 580 590 600
MRDEGLVSGD EPFERLLCQG MVNAESFFIK SEGKENWIEP ENVVIERDDK
610 620 630 640 650
GRFIAAKHKI TGDAVEFGGV IKMSKSKANG VDPESVINQY GADTVRLFTM
660 670 680 690 700
FAAPPEQSLE WSDSGVEGAS RFLRRLWKAV EGHINAGTPG KLDAASLPQQ
710 720 730 740 750
QKDLRRKTHE TIQKVSDDYG RRQTFNTAIA AVMELLNETS KLSDRANPQG
760 770 780 790 800
LAVEREALEA AILLLAPIVP HITQALWIEL GNSGIPLNQP WPTLDESALV
810 820 830 840 850
RSTIEVVVQV NGKLRGKIDA AVDAPKELLE QIAVQQENVQ KFLEGVTVRK
860
VIVVPNKLVN IVAN
Length:864
Mass (Da):97,610
Last modified:September 1, 2008 - v1
Checksum:iEBC31552266D6203
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE83294.1.
RefSeqiYP_001980977.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE83294; ACE83294; CJA_0454.
KEGGicja:CJA_0454.
PATRICi21324289. VBICelJap122165_0464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE83294.1.
RefSeqiYP_001980977.1. NC_010995.1.

3D structure databases

ProteinModelPortaliB3PIB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498211.CJA_0454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE83294; ACE83294; CJA_0454.
KEGGicja:CJA_0454.
PATRICi21324289. VBICelJap122165_0464.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-454-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.

Entry informationi

Entry nameiSYL_CELJU
AccessioniPrimary (citable) accession number: B3PIB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: September 1, 2008
Last modified: March 31, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.