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Protein

Biotin biosynthesis bifunctional protein BioHC

Gene

bioC

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl-L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway (By similarity).By similarity
The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters (By similarity).By similarity

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.
S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] = S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester.

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei72 – 721NucleophileBy similarity
Active sitei195 – 1951By similarity
Active sitei223 – 2231By similarity
Binding sitei223 – 2231SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-428-MONOMER.
UniPathwayiUPA00078.

Protein family/group databases

ESTHERicelju-b3pi89. BioH.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis bifunctional protein BioHC
Including the following 2 domains:
Carboxylesterase BioH (EC:3.1.1.1)
Alternative name(s):
Biotin synthesis protein BioH
Malonyl-[acyl-carrier protein] O-methyltransferase (EC:2.1.1.197)
Short name:
Malonyl-ACP O-methyltransferase
Alternative name(s):
Biotin synthesis protein BioC
Gene namesi
Name:bioC
Ordered Locus Names:CJA_0428
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCellvibrionalesCellvibrionaceaeCellvibrio
Proteomesi
  • UP000001036 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Biotin biosynthesis bifunctional protein BioHCPRO_0000412489Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_0428.

Structurei

3D structure databases

ProteinModelPortaliB3PI89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 224224CarboxylesteraseAdd
BLAST
Regioni134 – 1385Substrate bindingBy similarity
Regioni225 – 502278Malonyl-ACP O-methyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.Curated
In the C-terminal section; belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410626R. Bacteria.
COG0500. LUCA.
COG0596. LUCA.
HOGENOMiHOG000066290.
KOiK02169.
OMAiHAMERET.
OrthoDBiEOG6QRWCF.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.40.50.1820. 1 hit.
HAMAPiMF_00835. BioC.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR011814. BioC.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
PF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
SSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02072. BioC. 1 hit.

Sequencei

Sequence statusi: Complete.

B3PI89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPVLVLVH GWGCDSRTWQ PVLDGLRELV PVQLVDLPGF GNTPALETFS
60 70 80 90 100
LPAVLAAIES QLPERCVLLG WSLGAMLAVQ LAARLPQQVL GVISLAANAR
110 120 130 140 150
FVASDDYPHA MAPDVNRQFN SRFAEQPQAA LKMFTGLLAQ GDVQERALLK
160 170 180 190 200
QLRTQVPDAI NHNWAQALQL LAELDNRAAL VQLSQPLLHL LAEQDALVPI
210 220 230 240 250
AAAESLRGLN SQHQIHVIAG SAHAVHWSQP QQLISAVQDF YETLATAVDK
260 270 280 290 300
KRVAQSFGKA AATYDSVAGL QRAVGAQLLD YLPAQLDRTR LLDIGSGTGF
310 320 330 340 350
FTAQLATRGA EVIALDIAQG MLDFARQQHP QAADWVCGDA ENLPFAQSSV
360 370 380 390 400
DFIFSSLVIQ WCARVPQLMQ ELARVLKPGG RAYISTLGPG TLVELKRAWQ
410 420 430 440 450
QVDNYVHVNR FVGRTSLEQA VQQAGMQCLA FVESTRRLYF SRLRDLTHEL
460 470 480 490 500
KALGAHNINP GQAQGLTGRQ RLQAFSLAYE RERSPQGLPA SYEVYYLVLC

KP
Length:502
Mass (Da):55,021
Last modified:September 2, 2008 - v1
Checksum:i4F5D07B07B16B36B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE82969.1.
RefSeqiWP_012486110.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE82969; ACE82969; CJA_0428.
KEGGicja:CJA_0428.
PATRICi21324237. VBICelJap122165_0439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000934 Genomic DNA. Translation: ACE82969.1.
RefSeqiWP_012486110.1. NC_010995.1.

3D structure databases

ProteinModelPortaliB3PI89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498211.CJA_0428.

Protein family/group databases

ESTHERicelju-b3pi89. BioH.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE82969; ACE82969; CJA_0428.
KEGGicja:CJA_0428.
PATRICi21324237. VBICelJap122165_0439.

Phylogenomic databases

eggNOGiENOG410626R. Bacteria.
COG0500. LUCA.
COG0596. LUCA.
HOGENOMiHOG000066290.
KOiK02169.
OMAiHAMERET.
OrthoDBiEOG6QRWCF.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciCJAP498211:GHIT-428-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.40.50.1820. 1 hit.
HAMAPiMF_00835. BioC.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR011814. BioC.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
PF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
SSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02072. BioC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.

Entry informationi

Entry nameiBIOHC_CELJU
AccessioniPrimary (citable) accession number: B3PI89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 2, 2008
Last modified: May 11, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.