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B3PGN4 (GLGB_CELJU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:CJA_1885
OrganismCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]
Taxonomic identifier498211 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7377371,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000131812

Sites

Active site4191Nucleophile By similarity
Active site4721Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B3PGN4 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: AD82314B7066664B

FASTA73784,963
        10         20         30         40         50         60 
MSNPKQEQHL SQDLLRIITA THHDPFEVLG RHSLPVPTAT ADTLVRVYLP GARSAELVIN 

        70         80         90        100        110        120 
RQNRQIAPLN RLPGTDFFEW YGLGQQLPVH YQIEWYDRHN QHRVHYDPYA FAPQLGELDM 

       130        140        150        160        170        180 
HLFAEGQHWN IYNLLGAHPR QVDGINGVLF ATWAPNAERI SVVGNFNDWD GRHHPMRVRG 

       190        200        210        220        230        240 
SSGLWELFIP GVQPGDLYKF EIRNRQTGAV FLKSDPYGQS FEHRPSTSSI ITADSAFAWQ 

       250        260        270        280        290        300 
DADWMEHRAH WDWQASPLSI YEVHLGSWRR GWAGEFLSYR ELAHQLADYV KYMGFTHVEI 

       310        320        330        340        350        360 
LPVSEHPLDD SWGYQTTGYY APTSRFGSPD DFRYFVDHLH QQGIGIILDW VPAHFPKDAH 

       370        380        390        400        410        420 
ALARFDGSAL YEHEDPRLGE HRDWGTLIYN YGRSEVRNFL LANALFWLKE YHIDGLRVDA 

       430        440        450        460        470        480 
VASMLHLDYS RQPGEWIPNI HGGNENLEAM TFLQQLNAIC HGQHSGALVI AEESTAWPQV 

       490        500        510        520        530        540 
TRPTWVGGLG FSMKWNMGWM HDTLEYMSRE PVHRQYHHNQ LTFGMMYAFT ENFQLPFSHD 

       550        560        570        580        590        600 
EVVHGKGSMI NKMPGDDWQK FANLRLLYTY LYTYPGSKLL FMGGEFAQWS EWAHGRSLDW 

       610        620        630        640        650        660 
HLLDYGPHQG VQSAVKDLNH LYRQVPSLYQ RNFRGDGFEW IDCHDSTQSI ISYIRSSDSE 

       670        680        690        700        710        720 
FTLVILNFTP VPRYNYRIGV PVAGQYQEVF NSDSQFYGGS NLGNANPLYT QPVPWMNHGQ 

       730 
SLEVTLPPLG GLLLKLA 

« Hide

References

[1]"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ueda107.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000934 Genomic DNA. Translation: ACE85398.1.
RefSeqYP_001982359.1. NC_010995.1.

3D structure databases

ProteinModelPortalB3PGN4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498211.CJA_1885.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE85398; ACE85398; CJA_1885.
GeneID6413337.
KEGGcja:CJA_1885.
PATRIC21327160. VBICelJap122165_1859.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycCJAP498211:GHIT-1878-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_CELJU
AccessionPrimary (citable) accession number: B3PGN4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries