B3PGN4 (GLGB_CELJU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	CJA_1885

Organism

Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio › Cellvibrio japonicus

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_1000131812
Sites
	Active site	419	1	Nucleophile By similarity
	Active site	472	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PGN4 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: AD82314B7066664B	FASTA	737	84,963
	10 20 30 40 50 60 MSNPKQEQHL SQDLLRIITA THHDPFEVLG RHSLPVPTAT ADTLVRVYLP GARSAELVIN 70 80 90 100 110 120 RQNRQIAPLN RLPGTDFFEW YGLGQQLPVH YQIEWYDRHN QHRVHYDPYA FAPQLGELDM 130 140 150 160 170 180 HLFAEGQHWN IYNLLGAHPR QVDGINGVLF ATWAPNAERI SVVGNFNDWD GRHHPMRVRG 190 200 210 220 230 240 SSGLWELFIP GVQPGDLYKF EIRNRQTGAV FLKSDPYGQS FEHRPSTSSI ITADSAFAWQ 250 260 270 280 290 300 DADWMEHRAH WDWQASPLSI YEVHLGSWRR GWAGEFLSYR ELAHQLADYV KYMGFTHVEI 310 320 330 340 350 360 LPVSEHPLDD SWGYQTTGYY APTSRFGSPD DFRYFVDHLH QQGIGIILDW VPAHFPKDAH 370 380 390 400 410 420 ALARFDGSAL YEHEDPRLGE HRDWGTLIYN YGRSEVRNFL LANALFWLKE YHIDGLRVDA 430 440 450 460 470 480 VASMLHLDYS RQPGEWIPNI HGGNENLEAM TFLQQLNAIC HGQHSGALVI AEESTAWPQV 490 500 510 520 530 540 TRPTWVGGLG FSMKWNMGWM HDTLEYMSRE PVHRQYHHNQ LTFGMMYAFT ENFQLPFSHD 550 560 570 580 590 600 EVVHGKGSMI NKMPGDDWQK FANLRLLYTY LYTYPGSKLL FMGGEFAQWS EWAHGRSLDW 610 620 630 640 650 660 HLLDYGPHQG VQSAVKDLNH LYRQVPSLYQ RNFRGDGFEW IDCHDSTQSI ISYIRSSDSE 670 680 690 700 710 720 FTLVILNFTP VPRYNYRIGV PVAGQYQEVF NSDSQFYGGS NLGNANPLYT QPVPWMNHGQ 730 SLEVTLPPLG GLLLKLA

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ueda107.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000934 Genomic DNA. Translation: ACE85398.1.
RefSeq	YP_001982359.1. NC_010995.1.
3D structure databases
ProteinModelPortal	B3PGN4.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	498211.CJA_1885.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE85398; ACE85398; CJA_1885.
GeneID	6413337.
KEGG	cja:CJA_1885.
PATRIC	21327160. VBICelJap122165_1859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	GELSHAW.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
BioCyc	CJAP498211:GHIT-1878-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_CELJU

Accession

Primary (citable) accession number: B3PGN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program