Reviewed, UniProtKB/Swiss-Prot B3PEP6 (KATG2_CELJU)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	CJA_0025

Organism

Cellvibrio japonicus (strain Ueda107) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 740	713	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354756
Sites
	Active site	107	1	Proton acceptor By similarity
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Site	103	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	106 ↔ 228		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254) By similarity
	Cross-link	228 ↔ 254		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-106) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PEP6-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 2FD3F6FC1791C1DC	FASTA	740	81,550
	10 20 30 40 50 60 MFKKTKPRIS ILALTISCAI YSGAALAQDA ANSNKFWWPE QLNLSPLRQH GVESNPMGST 70 80 90 100 110 120 FNYAQAFKKL DLNAVKADIK ALMTQSQDWW PADYGHYGPF FIRMAWHSAG TYRIYDGRGG 130 140 150 160 170 180 AGGGQQRFEP LNSWPDNVNL DRARRLLWPI KQKYGSSISW ADLMVLTGNV ALESMGFKTF 190 200 210 220 230 240 GFAGGRQDDW EADTVYWGPE KKWLDDKRYS GDRTLEKPLA AVQMGLIYVN PEGPNGVPDP 250 260 270 280 290 300 LLAAKDIRDT FGRMAMNDEE TVALIAGGHT FGKAHGAHKP ETCLGKEPAA AGIEEQGLGW 310 320 330 340 350 360 TNKCGKGNAE DTITSGLEGA WSVNPIAWTT QYLDNLFAFE WVQVRSPAGA VQWIPKDGQA 370 380 390 400 410 420 ANLVPDAHDK TKRHAPIMFT TDLALKEDPE YRKISLRFKE NPKEFELAFA KAWFKLTHRD 430 440 450 460 470 480 MGPRVRYLGN EVPGEILLWQ DPVPEVDHPL IDAADITKLK SSLLSSGLSS AELVRTAWAA 490 500 510 520 530 540 AASFRGTDLR GGANGARIRL APQNTWAVNN PRELNRALTR LEKVQGDFNK ASTGGKKVSL 550 560 570 580 590 600 ADVIVLGGVA AVEQAAKKAG YAVEVPFIPG RTDASQAQTD VTSFAVLEPT ADGFRNYYAK 610 620 630 640 650 660 DNTLPPTDML VERANLLTLT VPEMTVLVGG LRVLGANSDA AKNGIFTDKP GTLSNDFFIN 670 680 690 700 710 720 LLDMSTQWRK SAKTGGLYEG LDRKTGKLKW TATPVDLIFG SHSELRAVAE VYAANDGQEK 730 740 FVNDFVKAWH KVMMLDRFDW

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000934 Genomic DNA. Translation: ACE85973.1.
RefSeq	YP_001980549.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6413225.
GenomeReviews	Gene locus CJA_0025 in contig CP000934_GR.
KEGG	cja:CJA_0025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_CELJU

Accession

Primary (citable) accession number: B3PEP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 2, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)