B3PE13 (LSPA_CELJU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	CJA_3216

Organism

Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190796
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
Sites
	Active site	114	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PE13 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: B20F4913C446AD10	FASTA	170	19,312
	10 20 30 40 50 60 MTKKVYHQYW FWYLAAFVVF LLDQGSKHWI EAAFDYNETK VFTSFFNFTL RYNPGAAFSF 70 80 90 100 110 120 LADAGGWQRW FFTIVAVAAS VLLIVWICRV ASSKPREAFA LSFILGGAVG NLYDRIIHGH 130 140 150 160 170 VVDFIVVHYQ DYYWPAFNLA DAAISLGAMV LIADLFINPD KTSGEKPTNA

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ueda107.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000934 Genomic DNA. Translation: ACE86004.1.
RefSeq	YP_001983670.1. NC_010995.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3PE13.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6416712.
GenomeReviews	Gene locus CJA_3216 in contig CP000934_GR.
KEGG	cja:CJA_3216.
PATRIC	21329829. VBICelJap122165_3179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
OMA	RGVSYGL.
Enzyme and pathway databases
BioCyc	CJAP155077:CJA_3216-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_CELJU

Accession

Primary (citable) accession number: B3PE13

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program