Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B3PCL8 (B3PCL8_CELJU) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:CJA_2927 EMBL ACE86079.1
OrganismCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP] EMBL ACE86079.1
Taxonomic identifier498211 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain121 – 316196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding147 – 20256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2831Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2831Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2851Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B3PCL8 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: D442F1084567B055

FASTA32835,220
        10         20         30         40         50         60 
MQAVKLPIDE TLKQQLGRVG VLYGGLSAER EISLQSGAAV IAALEDAGID HVAIDVGDNV 

        70         80         90        100        110        120 
IADIQAANID RAFLMLHGPG GEDGRIQALL EFLHIPYTGS DVQSSALAMD KLRTKFLWCG 

       130        140        150        160        170        180 
YSQSSAQHLP TPEFAVLTRE SDFVAELKRL GGEVMVKPAN EGSSIGMSRV KTAEDLQAAF 

       190        200        210        220        230        240 
VKATDYQGSV LAERLIVGAE YTVAILDGEA LPPIRLETDH SFYDFNAKYI AEDTRYICPC 

       250        260        270        280        290        300 
GLSAEKEQEL KQLALTAFNV LGCRGWGRVD VMADAQQNFY LLEVNTAPGM TSHSLVPMAA 

       310        320 
KAVGLSFTEL VVTILRASLV TAGSEVVA 

« Hide

References

[1]"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
Deboy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ueda107 EMBL ACE86079.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000934 Genomic DNA. Translation: ACE86079.1.
RefSeqYP_001983383.1. NC_010995.1.

3D structure databases

ProteinModelPortalB3PCL8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498211.CJA_2927.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE86079; ACE86079; CJA_2927.
GeneID6413973.
KEGGcja:CJA_2927.
PATRIC21329233. VBICelJap122165_2882.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCJAP498211:GHIT-2918-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3PCL8_CELJU
AccessionPrimary (citable) accession number: B3PCL8
Entry history
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)