Reviewed, UniProtKB/Swiss-Prot B3PC77 (KATG1_CELJU)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	CJA_2891

Organism

Cellvibrio japonicus (strain Ueda107) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354755
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PC77-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 73D3FD614B291706	FASTA	729	80,327
	10 20 30 40 50 60 MDNNPPTSTG KCPFTHGSVT ASGKSNTDWW PNALNLDILH QHDRKTNPLG DDFSYAAAFK 70 80 90 100 110 120 TLDLQAVKND LKALMTDSQD WWPADWGHYG GLFIRMAWHS AGSYRIADGR GGAGTGNQRF 130 140 150 160 170 180 APLNSWPDNG NLDKARRLLW PIKKKYGNKL SWADLMILAG NMAYESMGLK TFGFGGGRED 190 200 210 220 230 240 IWHPEKDVYW GSEKEWLAKS GGEGSRYSGE RDLENPLAAV MMGLIYVNPE GVDGNPDPLR 250 260 270 280 290 300 TAKDIRETFA RMAMNDEETV ALTAGGHTVG KAHGNGNAAN LGPDPEAADV EEQGLGWVNH 310 320 330 340 350 360 KTRGIGRDTV TSGIEGAWTT HPTKWDNGYF DMLLNHDWEL TKSPAGAWQW KPVSIKEEDM 370 380 390 400 410 420 PVDVEDPSIR CTPLMTDADM AMKMDPEYRK ISERFYSDPA YFSEVFARAW FKLTHRDLGP 430 440 450 460 470 480 KARYLGADVP AEDLIWQDPV PTVDYTLSDA DIADLKAKIL ASGLSVAELV ATAWDSARTF 490 500 510 520 530 540 RGSDFRGGAN GARIRLAPQK DWEGNEPARL QKVLGVFAGI QSGLSKKVSI ADLIVVGGAA 550 560 570 580 590 600 AVEKAARDAG VNITVPFAPG RGDATDAQTD AESFAPLEPV HDGFRNWVKK DYAVQPEEML 610 620 630 640 650 660 LDRAQLMGLT APEMTVLVGG MRVLGANYGG SKNGVFTDKV GVLSNDFFVN LTDMAYQWKP 670 680 690 700 710 720 TGKNSYNIVE RNTGTVKWSA TRVDLVFGSN SILRSYAEVY AQDDNREKFV RDFVKAWVKV MNADRFDLK

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000934 Genomic DNA. Translation: ACE86124.1.
RefSeq	YP_001983347.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6414538.
GenomeReviews	Gene locus CJA_2891 in contig CP000934_GR.
KEGG	cja:CJA_2891.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B3PC77. WPNALNL.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_CELJU

Accession

Primary (citable) accession number: B3PC77

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)