Reviewed, UniProtKB/Swiss-Prot B3PC19 (DAPA_CELJU)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	CJA_2830

Organism

Cellvibrio japonicus (strain Ueda107) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000124020
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PC19-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 3A041138AC9F2CA5	FASTA	290	30,994
	10 20 30 40 50 60 MIQGSMVALV TPMHADNTLD WDSLHKLVDW HLEQGTHAIV AVGTTGESAT LDVQEHLQVI 70 80 90 100 110 120 KRVVDQVNGR IPVIAGTGAN STSEAVELTQ AAKDVGADAC LLVTPYYNKP TQEGLFLHHE 130 140 150 160 170 180 YIANAVAIPQ YLYNVPGRTG VDMKPETALR LAQVPNIAGI KEATGDLERA RLLIDQAPPG 190 200 210 220 230 240 FAIISGDDAT AVELILLGGQ GDISVTANVV PAAIARMCEL ALAGKAEEAR TINTQLLPLH 250 260 270 280 290 TAMFVESNPI PVKWAVEQLG LIQSGIRLPL TRLSAQYHQQ VKTAMQLAGL

References

[1]

"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000934 Genomic DNA. Translation: ACE83136.1.
RefSeq	YP_001983289.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6416131.
GenomeReviews	Gene locus CJA_2830 in contig CP000934_GR.
KEGG	cja:CJA_2830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG358848.
OMA	QKGLIEH.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. Dihydrodipicolinate_synth. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CELJU

Accession

Primary (citable) accession number: B3PC19

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)