Reviewed, UniProtKB/Swiss-Prot B3P3K2 (PDE6_DROER)

Names and origin

Protein names

Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase
    EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GG21262

Organism

Drosophila erecta (Fruit fly)

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. UniProtKB Q9VFI9
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1128	1128	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363686
	Propeptide	1129 – 1131	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363687
Regions
	Domain	255 – 412	158	GAF 1
	Domain	444 – 625	182	GAF 2
Amino acid modifications
	Modified residue	1128	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1128	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B3P3K2-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: D63B518C430CAE35	FASTA	1,131	125,578
	10 20 30 40 50 60 MTDVSSPAGG AASPVEMATS SSSAATTLTN GANKTAISTA AGVAPGAVPG PGSAAIPASS 70 80 90 100 110 120 SSGNQVKLEH HHRQSNNNRP AATNRSSETK LRSPAGESDG ASRLMTPAGS SSTPSQSPSP 130 140 150 160 170 180 SQSPSQASIQ TQTSQQDRLA KASTTASQQD VDEVARLFEE KPEAFEKWLT ERAPPEALSR 190 200 210 220 230 240 LQEFIENRKP HKRPSVTSDL FQQWMAASPT VQQKSPRSLS NSSASSIPEC RRHLMDLDEG 250 260 270 280 290 300 ELFMELIRDV ANELDIDVLC HKILVNVGLL THADRGSLFL AKGTPTNKYL VAKLFDVTQK 310 320 330 340 350 360 TALKDAVTRA SAEEIIIPFG IGIAGMVAQT KQMINIKEAY KDARFNCEID LKTGYKTNAI 370 380 390 400 410 420 LCMPICNYEG DIIGVAQIIN KTNGGCFKGC MEFDEHDVEI FRRYLTFCGI GIQNAQLFEM 430 440 450 460 470 480 SVQEYRRNQI LLNLARSIFE EQNNLECLVT KIMTEARELL KCERCSVFLV DLDCCEASHL 490 500 510 520 530 540 EKIIEKPNQP ATRAIKSADS FEEKKMRNRF TVLFELGGEY QAANVSRPSV SELSSSTLAQ 550 560 570 580 590 600 IAQFVATTGQ TVNICDVIEW VRDHNQIRAE DEIDSTQAIL CMPIMNAQKK VIGVAQLINK 610 620 630 640 650 660 ANGVPFTDSD ASIFEAFAIF CGLGIHNTQM YENACKLMAK QKVALECLSY HATASQDQTE 670 680 690 700 710 720 KLTQDVIAEA ESYNLYSFTF TDFELVDDDT CRAVLRMFMQ CNLVSQFQIP YDVLCRWVLS 730 740 750 760 770 780 VRKNYRPVKY HNWRHALNVA QTMFAMLKTG KMERFMTDLE ILGLLVACLC HDLDHRGTNN 790 800 810 820 830 840 AFQTKTESPL AILYTTSTME HHHFDQCVMI LNSEGNNIFQ ALSPEDYRSV MKTVESAILS 850 860 870 880 890 900 TDLAMYFKKR NAFLELVENG EFDWQGEEKK DLLCGMMMTA CDVSAIAKPW EVQHKVAKLV 910 920 930 940 950 960 ADEFFDQGDL EKLQLNTQPV AMMDRERKDE LPKMQVGFID VICLPLYRVL CDTFPWITPL 970 980 990 1000 1010 1020 YEGTLENRRN WQDLAEKVEM GLTWIDHDTI DKPVEEFAAC ADEEIKDIEF TVTTLNCNQQ 1030 1040 1050 1060 1070 1080 SQHGSEDSHT PEHQRSGSRL SMKKTGALGK AVRSKLSKTL YNSMDGSKPK TSLKLLESHV 1090 1100 1110 1120 1130 SEDMDDKSPT SPSQPQASGS MGRMSASSST SSAGGQMVDK SKKRSKLCAL L

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14021-0224.01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH954181 Genomic DNA. Translation: EDV48890.1.
RefSeq	XP_001979932.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6552139.
KEGG	der:Dere_GG21262.
Organism-specific databases
FlyBase	FBgn0113442. Dere\GG21262.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROER

Accession

Primary (citable) accession number: B3P3K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 2, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project