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B3P3K2 (PDE6_DROER) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase

EC=3.1.4.35
Gene names
Name:Pde6
ORF Names:GG21262
OrganismDrosophila erecta (Fruit fly) [Complete proteome]
Taxonomic identifier7220 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Interacts with PrBP By similarity. UniProtKB Q9VFI9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11281128cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9
PRO_0000363686
Propeptide1129 – 11313Removed in mature form By similarity UniProtKB Q9VFI9
PRO_0000363687

Regions

Domain255 – 412158GAF 1
Domain444 – 625182GAF 2

Sites

Active site7311Proton donor By similarity
Metal binding7351Divalent metal cation 1 By similarity
Metal binding7711Divalent metal cation 1 By similarity
Metal binding7721Divalent metal cation 1 By similarity
Metal binding7721Divalent metal cation 2 By similarity
Metal binding8821Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue11281Cysteine methyl ester By similarity UniProtKB Q9VFI9
Lipidation11281S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

Sequence LengthMass (Da)Tools
B3P3K2 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: D63B518C430CAE35

FASTA1,131125,578
        10         20         30         40         50         60 
MTDVSSPAGG AASPVEMATS SSSAATTLTN GANKTAISTA AGVAPGAVPG PGSAAIPASS 

        70         80         90        100        110        120 
SSGNQVKLEH HHRQSNNNRP AATNRSSETK LRSPAGESDG ASRLMTPAGS SSTPSQSPSP 

       130        140        150        160        170        180 
SQSPSQASIQ TQTSQQDRLA KASTTASQQD VDEVARLFEE KPEAFEKWLT ERAPPEALSR 

       190        200        210        220        230        240 
LQEFIENRKP HKRPSVTSDL FQQWMAASPT VQQKSPRSLS NSSASSIPEC RRHLMDLDEG 

       250        260        270        280        290        300 
ELFMELIRDV ANELDIDVLC HKILVNVGLL THADRGSLFL AKGTPTNKYL VAKLFDVTQK 

       310        320        330        340        350        360 
TALKDAVTRA SAEEIIIPFG IGIAGMVAQT KQMINIKEAY KDARFNCEID LKTGYKTNAI 

       370        380        390        400        410        420 
LCMPICNYEG DIIGVAQIIN KTNGGCFKGC MEFDEHDVEI FRRYLTFCGI GIQNAQLFEM 

       430        440        450        460        470        480 
SVQEYRRNQI LLNLARSIFE EQNNLECLVT KIMTEARELL KCERCSVFLV DLDCCEASHL 

       490        500        510        520        530        540 
EKIIEKPNQP ATRAIKSADS FEEKKMRNRF TVLFELGGEY QAANVSRPSV SELSSSTLAQ 

       550        560        570        580        590        600 
IAQFVATTGQ TVNICDVIEW VRDHNQIRAE DEIDSTQAIL CMPIMNAQKK VIGVAQLINK 

       610        620        630        640        650        660 
ANGVPFTDSD ASIFEAFAIF CGLGIHNTQM YENACKLMAK QKVALECLSY HATASQDQTE 

       670        680        690        700        710        720 
KLTQDVIAEA ESYNLYSFTF TDFELVDDDT CRAVLRMFMQ CNLVSQFQIP YDVLCRWVLS 

       730        740        750        760        770        780 
VRKNYRPVKY HNWRHALNVA QTMFAMLKTG KMERFMTDLE ILGLLVACLC HDLDHRGTNN 

       790        800        810        820        830        840 
AFQTKTESPL AILYTTSTME HHHFDQCVMI LNSEGNNIFQ ALSPEDYRSV MKTVESAILS 

       850        860        870        880        890        900 
TDLAMYFKKR NAFLELVENG EFDWQGEEKK DLLCGMMMTA CDVSAIAKPW EVQHKVAKLV 

       910        920        930        940        950        960 
ADEFFDQGDL EKLQLNTQPV AMMDRERKDE LPKMQVGFID VICLPLYRVL CDTFPWITPL 

       970        980        990       1000       1010       1020 
YEGTLENRRN WQDLAEKVEM GLTWIDHDTI DKPVEEFAAC ADEEIKDIEF TVTTLNCNQQ 

      1030       1040       1050       1060       1070       1080 
SQHGSEDSHT PEHQRSGSRL SMKKTGALGK AVRSKLSKTL YNSMDGSKPK TSLKLLESHV 

      1090       1100       1110       1120       1130 
SEDMDDKSPT SPSQPQASGS MGRMSASSST SSAGGQMVDK SKKRSKLCAL L 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 14021-0224.01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH954181 Genomic DNA. Translation: EDV48890.1.
RefSeqXP_001979932.1. XM_001979896.1.

3D structure databases

ProteinModelPortalB3P3K2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0141316; FBpp0139808; FBgn0113442.
GeneID6552139.
KEGGder:Dere_GG21262.

Organism-specific databases

FlyBaseFBgn0113442. Dere\GG21262.

Phylogenomic databases

KOK13763.
OrthoDBEOG7RRF69.
PhylomeDBB3P3K2.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE6_DROER
AccessionPrimary (citable) accession number: B3P3K2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase