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B3NIL9

- LIAS_DROER

UniProt

B3NIL9 - LIAS_DROER

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Protein

Lipoyl synthase, mitochondrial

Gene
Las, GG13311
Organism
Drosophila erecta (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:Las
ORF Names:GG13311
OrganismiDrosophila erecta (Fruit fly)
Taxonomic identifieri7220 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000008711: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0105583. Dere\GG13311.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 379Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398217
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Structurei

3D structure databases

ProteinModelPortaliB3NIL9.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiB3NIL9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3NIL9-1 [UniParc]FASTAAdd to Basket

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MFGMLRALKT HVEAPIVVAT RAVSTNAEKL EEIRERLAKG PNFQDFVQNP    50
ENSRSEWEKY EGKLRREKGE EQRLRLPPWL KTTIPVGKNY AKIKAQMREL 100
KLSTVCEEAR CPNIGECWGG GEHGTQTATI MLMGDTCTRG CRFCSVKTAR 150
RPPPLDVNEP VNTATAIASW GLDYIVLTSV DRDDLPDGGS KHIAETVREI 200
KARNSNIFVE CLVPDFRGNL ECVETIANSG LDVYAHNIET VEKLTPYVRD 250
RRAHYRQTLQ VLTEAKRFNP NLITKSSIML GLGETDGEIE CTLKDLREAG 300
VDCVTLGQYM QPTNKHLKVI EYVTPEKFKH WEERGNALGF LYTASGPLVR 350
SSYKAGEFFI TSILENRKKR QNATEIAKE 379
Length:379
Mass (Da):42,807
Last modified:September 2, 2008 - v1
Checksum:i59480438DFBEB65A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH954178 Genomic DNA. Translation: EDV52515.1.
RefSeqiXP_001973489.1. XM_001973453.1.

Genome annotation databases

EnsemblMetazoaiFBtr0133365; FBpp0131857; FBgn0105583.
GeneIDi6545884.
KEGGider:Dere_GG13311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH954178 Genomic DNA. Translation: EDV52515.1 .
RefSeqi XP_001973489.1. XM_001973453.1.

3D structure databases

ProteinModelPortali B3NIL9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0133365 ; FBpp0131857 ; FBgn0105583 .
GeneIDi 6545884.
KEGGi der:Dere_GG13311.

Organism-specific databases

FlyBasei FBgn0105583. Dere\GG13311.

Phylogenomic databases

KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi B3NIL9.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 14021-0224.01.

Entry informationi

Entry nameiLIAS_DROER
AccessioniPrimary (citable) accession number: B3NIL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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