Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B3M996

- LIAS_DROAN

UniProt

B3M996 - LIAS_DROAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LasUniRule annotation
ORF Names:GF24134
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000007801: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0101128. Dana\GF24134.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 376Lipoyl synthase, mitochondrialPRO_0000398216
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi7217.FBpp0127326.

Structurei

3D structure databases

ProteinModelPortaliB3M996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiB3M996.
KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiB3M996.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3M996-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRALKGHVE SPLIVVTRAA STNAEKLEEI RERLAKGPNF QDFVQNPDYS
60 70 80 90 100
KSEWENYEGK LRREKGEEQR LRLPPWLKTT IPMGKNYAKI KNQLRELKLS
110 120 130 140 150
TVCEEARCPN IGECWGGGEH GTQTATIMLM GDTCTRGCRF CSVKTARKPP
160 170 180 190 200
PLDENEPVNT ATAIASWGLD YIVLTSVDRD DLPDGGSKHI AKTVKEIKAR
210 220 230 240 250
NSNIFVECLV PDFRGDLGCV ETIANCGLDV YAHNIETVEK LTPYVRDRRA
260 270 280 290 300
HYRQTLKVLS EAKRFNPNLI TKSSIMLGLG ETDEEVENTL KDLREAGVDC
310 320 330 340 350
ITLGQYMQPT NKHLKVIEYV TPEKFKHWED RGNQLGFLYT ASGPLVRSSY
360 370
KAGEFFITSI LENRKKRQAL NSKPEQ
Length:376
Mass (Da):42,549
Last modified:September 2, 2008 - v1
Checksum:i49136A35750CDB7C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH902618 Genomic DNA. Translation: EDV40080.1.
RefSeqiXP_001957274.1. XM_001957238.1.

Genome annotation databases

EnsemblMetazoaiFBtr0128834; FBpp0127326; FBgn0101128.
GeneIDi6506768.
KEGGidan:Dana_GF24134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH902618 Genomic DNA. Translation: EDV40080.1 .
RefSeqi XP_001957274.1. XM_001957238.1.

3D structure databases

ProteinModelPortali B3M996.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7217.FBpp0127326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0128834 ; FBpp0127326 ; FBgn0101128 .
GeneIDi 6506768.
KEGGi dan:Dana_GF24134.

Organism-specific databases

FlyBasei FBgn0101128. Dana\GF24134.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi B3M996.
KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi B3M996.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 14024-0371.13.

Entry informationi

Entry nameiLIAS_DROAN
AccessioniPrimary (citable) accession number: B3M996
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3