Spastin - Drosophila ananassae (Fruit fly)

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Reviewed, UniProtKB/Swiss-Prot B3M301 (SPAST_DROAN)

Last modified October 13, 2009. Version 12. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	GF17034

Organism

Drosophila ananassae (Fruit fly)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

770

Spastin

PRO_0000367141

Regions

Transmembrane

20

Potential

Domain

77

MIT

Nucleotide binding

8

ATP Potential

Region

213

Required for localization to punctate cytoplasmic foci By similarity

Region

560

Sufficient for interaction with microtubules and microtubule severing By similarity

Region

15

Required for interaction with microtubules By similarity

Amino acid modifications

Modified residue

1

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

B3M301-1 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: CBA1DB86D6A95829
Show »

770

83,796

        10         20         30         40         50         60 
MVRTKNQSSS SSASSSTKSP IKSGSAGSGS AAGGNSASGS RQSTHRSSSA SNVASAAAAA 

        70         80         90        100        110        120 
VASSNRTRTS PGSSPDGDDD TTTTDDLTPT SCSPRSGHHH HGHPYGGSSV HKQNLYVVSF 

       130        140        150        160        170        180 
PIIFLFNVLR SLIYQLFCIF RYLYGASTKV IYRPHRRDCN IEIVVQNSKD QQQQHQSQPL 

       190        200        210        220        230        240 
SYPLELSEGG NPEQQLPSQT QRYRAIQPLE MASNRPGGGY SPGPGDPLLA KQKHHHRRAF 

       250        260        270        280        290        300 
EYISKALKID EENEGHKELA IELYRKGIKE LEDGIAVDCW SGRGDVWDRA QRLHDKMQTN 

       310        320        330        340        350        360 
LSMARDRLHF LALREEDLRM QRLSLKEKQQ KTLPQSDYKA LKSREPMLAG MTNDPLKPRV 

       370        380        390        400        410        420 
RSSGYGPKAS TSAQAAAATA ASGRKLTIGT KRPGNLAVAN KSQTLPRNLG SKTSVGAVQR 

       430        440        450        460        470        480 
QPAKTAATPP AVRRQFSSGR NTPPLRSRTP INNNGPSTSG SGASTPVVSV KGVEPKLVQL 

       490        500        510        520        530        540 
ILDEIVEGGA KVEWSDIAGQ EVAKQALQEM VILPSVRPEL FTGLRAPAKG LLLFGPPGNG 

       550        560        570        580        590        600 
KTLLARAVAT ECSATFLNIS AASLTSKYVG DGEKLVRALF AVARHMQPSI IFIDEVDSLL 

       610        620        630        640        650        660 
SERSSNEHEA SRRLKTEFLV EFDGLPGNPD GDRIVVLAAT NRPQELDEAA LRRFTKRVYV 

       670        680        690        700        710        720 
SLPDEQTREL LLSRLLQKQG SPLDTEALRR LAKTTDGYSG SDLTALAKDA ALEPIRELNV 

       730        740        750        760        770 
EQVKCLDISA MRAITESDFH SSLKRIRRSV APQSLNSYEK WSQDYGDITI

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14024-0371.13.

Cross-references

Sequence databases
	CH902617 Genomic DNA. Translation: EDV42401.1.
RefSeq	XP_001953840.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6499823.
KEGG	dan:Dana_GF17034.
Organism-specific databases
FlyBase	FBgn0094053. Dana\GF17034.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DROAN

Accession

Primary (citable) accession number: B3M301

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	October 13, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project

Relevant documents

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents