B3LXF2 (DGKH_DROAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Gene names

ORF Names:

GF18176

Organism

Drosophila ananassae (Fruit fly) [Complete proteome]

Taxonomic identifier

7217 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1916 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity UniProtKB Q86XP1.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence EDV42796.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1916	1916	Diacylglycerol kinase eta		PRO_0000375984
Regions
	Domain	82 – 175	94	PH
	Domain	350 – 486	137	DAGKc
	Domain	1853 – 1916	64	SAM
	Zinc finger	195 – 245	51	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 319	52	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	B3LXF2 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: E49E3E9A4D283BA2	FASTA	1,916	213,112
	10 20 30 40 50 60 MAHIKLDTLD VVQRPGTTRR SNSNSGRSSA CSSGSLSPVP IIPIIAISRD TDDSESESEI 70 80 90 100 110 120 ETEPARLFQR RMSIKCTNNL AAIIKEGFLL KHTWSFQRWR RRYFRLKRNV LFHAKDFQCD 130 140 150 160 170 180 VLDEIDLSDL CYFECGIKNV NHSSQIITPT RSLVLCAESR REMEDWLGSL KTATTPQRPR 190 200 210 220 230 240 GDSFLIEQHD ILSNHHHWYA TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK 250 260 270 280 290 300 SIANCKWTTL ASVGKDIIEQ ADGSIIMPHQ WMEGNLPVSS VCAVCKKTCG SVLRLQDWRC 310 320 330 340 350 360 LWCRATVHVA CRPQMAVACP IGPAKLSVVP PTSVHSISTD DAWDVASPKG NFSPLLVFVN 370 380 390 400 410 420 SKSGDNQGVK FLRRFKQLLN PAQVFDLIST GPSLGLRLFR HFEMFRILVC SGDGSVGWVL 430 440 450 460 470 480 SEIDRFNMHK QCQVAVMPLG TGNDLARVLG WGSSCDDDTH LPQILERYES ASTKMLDRWS 490 500 510 520 530 540 IMVFEKAIAV PKTPKMSITT EQEALLTGMV TSANHHLRFI VETNDTQTLI SSTQSLCDTI 550 560 570 580 590 600 DDLVGRISEH HREDEQLAVK CDILRQKLNM LLDALQEEEM GAHSGDDLIA TIRSLIARSI 610 620 630 640 650 660 PQTPGTSAPL LNPSISIEKN EKDEINTKER RSSRSLRSSE KEALQSRANS VKRAIYNVVE 670 680 690 700 710 720 HSEPGRPKRY QRKLSITPFE ALKLPTTASG DSTPCSSPLP IIPPINIISP TMETSRLTCI 730 740 750 760 770 780 SPLPDTRRDS VDESFFNSIS LPAPRQFADS RRSSGVPDVI QEIEEGANGE TVYRIGRMSL 790 800 810 820 830 840 SGGANIDDAG NRLSPCSDGG ENTPTERKVD FLRVPILTGE PIVDPLSDYR PIEVFERTYY 850 860 870 880 890 900 MNRELDRGKE GTEEKKGDIE KEKSSGTDVE KEDNMPTEKQ ALVHICNLQV PGIVVTPNSQ 910 920 930 940 950 960 NVYTSASLTI IDTDAQTTNE QSSSEEIAGE ASDVLSAISN EECSVASEIF DKQDAGHTLG 970 980 990 1000 1010 1020 DIIQSLDASN FTHIDSPETS DETEAMPGES IMDDISSVLG HDITYALQDN TLTDDTTTLC 1030 1040 1050 1060 1070 1080 SEHVGPPKPP RKKSLSALTR GHSHPRRRNS SPPRIPRLAR MDSDDNPQQF GFENIVFEID 1090 1100 1110 1120 1130 1140 NRCDDQKMRE PPRYCSLAQF VEGNDIARQS FKQLMLEQHG SNDNDTEYPE QQTPTNTMTN 1150 1160 1170 1180 1190 1200 LMATTSEDEL STQTAIKIEI QDVDATMVRN INSSMKANTI LTTSTSPTKK SGHGQDISVV 1210 1220 1230 1240 1250 1260 VRPPTPLRGD SVKPSGSLLL DGSGGAISMA MGCSSLLGVR AMNAEIRRHS SHAPGLAVRE 1270 1280 1290 1300 1310 1320 IDKDKDRRHS GFNPNLLTLD PEHARFLSSS PAASRRISCG SLFKKNQKIA TKRGYGLFSV 1330 1340 1350 1360 1370 1380 RFLVVAEPDI RLATLALIRP LIPLPNEALP NLQTLKGSKS SLFMGSTLFG FDQFAAGDKE 1390 1400 1410 1420 1430 1440 KDEKGCKDKE KTPTEETGRK LPIINPLVRL PNWPNLANGG GFISKCLLAN ADTLCAAVSP 1450 1460 1470 1480 1490 1500 LMDPDETLLA GYHEKCVMNN YFGIGIDAKI SLDFHNKREE HPEKCRSRAR NYMWYGVLGS 1510 1520 1530 1540 1550 1560 KQLLQKTCKN LEQRVQLECD GQRIPLPELQ GIVILNIPSF MGGTNFWGSS TKKDDIFLPP 1570 1580 1590 1600 1610 1620 SFDDRVLEVV AVFGSVQMAA SRLINLQHHR IAQCQSVQIN ILGDEEIPIQ VDGEAWLQPP 1630 1640 1650 1660 1670 1680 GMIRILHKNR VQMLCRNRSL ELSLKSWQEK QRQHSISIQR DTSSTTSEHA TSTDEVISER 1690 1700 1710 1720 1730 1740 ECYVLLNFIE AVSSLVKWVK FLIISHPALQ HDLYAVACRA SEALESIHPQ GKLLEGPSLR 1750 1760 1770 1780 1790 1800 TKLVEVIDSS RQLYDDACTL LRDRGHSLIL REDLETKLSA ALANMEMELK KCSVQKCIDG 1810 1820 1830 1840 1850 1860 KLRAYFNVLA PNEEPDARRK SRPFWVRLRS GSTAGQQAFK PPLTNTREAA NNWSVNEVVT 1870 1880 1890 1900 1910 WLETMQLSEY VDSFLKNDIR GKELITLGRR DLKDLGVVKV GHVKRILQAI KDLSEN

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14024-0371.13.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH902617 Genomic DNA. Translation: EDV42796.1. Sequence problems.
RefSeq	XP_001954235.1. XM_001954199.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3LXF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6500954.
KEGG	dan:Dana_GF18176.
Organism-specific databases
FlyBase	FBgn0095194. Dana\GF18176.
Phylogenomic databases
GeneTree	EMGT00050000007391.
InParanoid	B3LXF2.
OrthoDB	EOG4280GF.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
KO	K00901.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROAN

Accession

Primary (citable) accession number: B3LXF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project