Reviewed, UniProtKB/Swiss-Prot B3LRX9 (ENOPH_YEAS1)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase
    Unknown transcript 4 protein

Gene names

Name:	UTR4
ORF Names:	SCRG_04432

Organism

Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)

Taxonomic identifier

285006 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that enolizes the substrate to form the intermediate 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Enolase-phosphatase E1		PRO_0000377656
Regions
	Region	118 – 119	2	Substrate binding By similarity
Sites
	Metal binding	11	1	Magnesium By similarity
	Metal binding	13	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	186	1	Magnesium By similarity
	Binding site	161	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3LRX9-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: E29DD481FD450D49	FASTA	227	25,208
	10 20 30 40 50 60 MGDNYSTYLL DIEGTVCPIS FVKETLFPYF TKKVPQLVQQ DTRDSPVSNI LSQFHIDDKE 70 80 90 100 110 120 QLQAHILELV AKDVKDPILK QLQGYIWAQG YESGQIKAPV YADAIDFIKR KKRVFIYSSG 130 140 150 160 170 180 SVKAQKLLFG YVQDPNAPAH DSLDLNSYID GYFDINTSGK KTETQSYANI LRDIGAKASE 190 200 210 220 VLFLSDNPLE LDAAAGVGIA TGLASRPGNA PVPDGQKYQV YKDFETL

References

[1]

"Annotation of the Saccharomyces cerevisiae RM11-1a genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Cuomo C., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., MacCallum I.A., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., O'Leary S., Kodira C.D., Zeng Q., Yandava C., Alvarado L., Pratt S., Kruglyak L. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH408052 Genomic DNA. Translation: EDV08795.1. Different initiation.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. IPR006439. HAD-SF_hydro_IA_v1. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit. TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENOPH_YEAS1

Accession

Primary (citable) accession number: B3LRX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	October 13, 2009
This is version 7 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)