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B3L8F2

- LIPA_PLAKH

UniProt

B3L8F2 - LIPA_PLAKH

Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Plasmodium knowlesi (strain H)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi177 – 1771Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi182 – 1821Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi188 – 1881Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi207 – 2071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi210 – 2101Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, apicoplastUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    ORF Names:PKH_120980
    OrganismiPlasmodium knowlesi (strain H)
    Taxonomic identifieri5851 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Plasmodium)
    ProteomesiUP000000622: Chromosome 12

    Organism-specific databases

    EuPathDBiPlasmoDB:PKH_120980.

    Subcellular locationi

    Plastidapicoplast UniRule annotation

    GO - Cellular componenti

    1. apicoplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apicoplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323UniRule annotationAdd
    BLAST
    Chaini24 – 442419Lipoyl synthase, apicoplastPRO_0000398231Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5850.PKH_120980.

    Structurei

    3D structure databases

    ProteinModelPortaliB3L8F2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B3L8F2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLTPSLYI YAFFIFCVRF KCGNRTTVAS AIRASYDMPP KELRVGDMLK    50
    KTSQPNCNYR TRGKCRKFFF VWKLDKMRDA HLGVQAKRRK NHLRSGSATY 100
    EASLGEHQLK GKRKESATNV EKEKKEKEQQ EERLPVPKVG NKMPEKKPDW 150
    FHVPAPTGKK YNKLKEDLKK LKLHTVCEEA QCPNIGECWN IGTATIMLLG 200
    DTCTRGCKFC SIKTSSKPLA PDANEPFNTA KAICEWDINY VVLTSVDRDD 250
    LPDGGASHFA KTIELIKFSR PEILIECLVS DFQGNVDSIR KLANSGMEVY 300
    AHNIETVRRL QKFVRDRRAN YEQSLRVLKI AKEINPMLYT KTSIMLGLGE 350
    TKEEVLEAMS DVRQHNIDVI TFGQYLRPTK NHLNVVEYVS PQMFDFYKEE 400
    GMKMGFKYIA SGPLVRSSYK AGEYFMKSLV EQRRGAKTHA QG 442
    Length:442
    Mass (Da):50,641
    Last modified:September 2, 2008 - v1
    Checksum:i4295AD91FB6F56A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM910994 Genomic DNA. Translation: CAQ41175.1.
    RefSeqiXP_002259908.1. XM_002259872.1.

    Genome annotation databases

    EnsemblProtistsiPKH_120980; PKH_120980; PKH_120980.
    GeneIDi7322246.
    KEGGipkn:PKH_120980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM910994 Genomic DNA. Translation: CAQ41175.1 .
    RefSeqi XP_002259908.1. XM_002259872.1.

    3D structure databases

    ProteinModelPortali B3L8F2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5850.PKH_120980.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi PKH_120980 ; PKH_120980 ; PKH_120980 .
    GeneIDi 7322246.
    KEGGi pkn:PKH_120980.

    Organism-specific databases

    EuPathDBi PlasmoDB:PKH_120980.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H.

    Entry informationi

    Entry nameiLIPA_PLAKH
    AccessioniPrimary (citable) accession number: B3L8F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3