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B3L8F2

- LIPA_PLAKH

UniProt

B3L8F2 - LIPA_PLAKH

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Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Plasmodium knowlesi (strain H)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi177 – 1771Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi182 – 1821Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi188 – 1881Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi203 – 2031Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi207 – 2071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi210 – 2101Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplastUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:lipAUniRule annotation
ORF Names:PKH_120980
OrganismiPlasmodium knowlesi (strain H)
Taxonomic identifieri5851 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Plasmodium)
ProteomesiUP000000622: Chromosome 12

Organism-specific databases

EuPathDBiPlasmoDB:PKH_120980.

Subcellular locationi

Plastidapicoplast UniRule annotation

GO - Cellular componenti

  1. apicoplast Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323UniRule annotationAdd
BLAST
Chaini24 – 442419Lipoyl synthase, apicoplastPRO_0000398231Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5850.PKH_120980.

Structurei

3D structure databases

ProteinModelPortaliB3L8F2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
InParanoidiB3L8F2.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3L8F2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVLTPSLYI YAFFIFCVRF KCGNRTTVAS AIRASYDMPP KELRVGDMLK
60 70 80 90 100
KTSQPNCNYR TRGKCRKFFF VWKLDKMRDA HLGVQAKRRK NHLRSGSATY
110 120 130 140 150
EASLGEHQLK GKRKESATNV EKEKKEKEQQ EERLPVPKVG NKMPEKKPDW
160 170 180 190 200
FHVPAPTGKK YNKLKEDLKK LKLHTVCEEA QCPNIGECWN IGTATIMLLG
210 220 230 240 250
DTCTRGCKFC SIKTSSKPLA PDANEPFNTA KAICEWDINY VVLTSVDRDD
260 270 280 290 300
LPDGGASHFA KTIELIKFSR PEILIECLVS DFQGNVDSIR KLANSGMEVY
310 320 330 340 350
AHNIETVRRL QKFVRDRRAN YEQSLRVLKI AKEINPMLYT KTSIMLGLGE
360 370 380 390 400
TKEEVLEAMS DVRQHNIDVI TFGQYLRPTK NHLNVVEYVS PQMFDFYKEE
410 420 430 440
GMKMGFKYIA SGPLVRSSYK AGEYFMKSLV EQRRGAKTHA QG
Length:442
Mass (Da):50,641
Last modified:September 2, 2008 - v1
Checksum:i4295AD91FB6F56A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM910994 Genomic DNA. Translation: CAQ41175.1.
RefSeqiXP_002259908.1. XM_002259872.1.

Genome annotation databases

EnsemblProtistsiPKH_120980; PKH_120980; PKH_120980.
GeneIDi7322246.
KEGGipkn:PKH_120980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM910994 Genomic DNA. Translation: CAQ41175.1 .
RefSeqi XP_002259908.1. XM_002259872.1.

3D structure databases

ProteinModelPortali B3L8F2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5850.PKH_120980.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi PKH_120980 ; PKH_120980 ; PKH_120980 .
GeneIDi 7322246.
KEGGi pkn:PKH_120980.

Organism-specific databases

EuPathDBi PlasmoDB:PKH_120980.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
InParanoidi B3L8F2.
KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H.

Entry informationi

Entry nameiLIPA_PLAKH
AccessioniPrimary (citable) accession number: B3L8F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 2, 2008
Last modified: November 26, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3