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Protein

Lipoyl synthase, apicoplast

Gene

lipA

Organism
Plasmodium knowlesi (strain H)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, apicoplast (lipA), Lipoyl synthase, apicoplast (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi182Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi188Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi203Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi207Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi210Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, apicoplastUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:lipAUniRule annotation
ORF Names:PKH_120980
OrganismiPlasmodium knowlesi (strain H)
Taxonomic identifieri5851 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Plasmodium)
Proteomesi
  • UP000031513 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiPlasmoDB:PKNH_1256700

Subcellular locationi

  • apicoplast UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23UniRule annotationAdd BLAST23
ChainiPRO_000039823124 – 442Lipoyl synthase, apicoplastAdd BLAST419

Interactioni

Protein-protein interaction databases

STRINGi5850.PKH_120980

Structurei

3D structure databases

ProteinModelPortaliB3L8F2
SMRiB3L8F2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000235998
InParanoidiB3L8F2
KOiK03644

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3L8F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLTPSLYI YAFFIFCVRF KCGNRTTVAS AIRASYDMPP KELRVGDMLK
60 70 80 90 100
KTSQPNCNYR TRGKCRKFFF VWKLDKMRDA HLGVQAKRRK NHLRSGSATY
110 120 130 140 150
EASLGEHQLK GKRKESATNV EKEKKEKEQQ EERLPVPKVG NKMPEKKPDW
160 170 180 190 200
FHVPAPTGKK YNKLKEDLKK LKLHTVCEEA QCPNIGECWN IGTATIMLLG
210 220 230 240 250
DTCTRGCKFC SIKTSSKPLA PDANEPFNTA KAICEWDINY VVLTSVDRDD
260 270 280 290 300
LPDGGASHFA KTIELIKFSR PEILIECLVS DFQGNVDSIR KLANSGMEVY
310 320 330 340 350
AHNIETVRRL QKFVRDRRAN YEQSLRVLKI AKEINPMLYT KTSIMLGLGE
360 370 380 390 400
TKEEVLEAMS DVRQHNIDVI TFGQYLRPTK NHLNVVEYVS PQMFDFYKEE
410 420 430 440
GMKMGFKYIA SGPLVRSSYK AGEYFMKSLV EQRRGAKTHA QG
Length:442
Mass (Da):50,641
Last modified:September 2, 2008 - v1
Checksum:i4295AD91FB6F56A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM910994 Genomic DNA Translation: CAQ41175.1
RefSeqiXP_002259908.1, XM_002259872.1

Genome annotation databases

EnsemblProtistsiCAQ41175; CAQ41175; PKH_120980
GeneIDi7322246
KEGGipkn:PKH_120980

Similar proteinsi

Entry informationi

Entry nameiLIPA_PLAKH
AccessioniPrimary (citable) accession number: B3L8F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 2, 2008
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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