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Protein

Adenylosuccinate synthetase

Gene
N/A
Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase, Adenylosuccinate synthetase isozyme 2 (ADSS), Adenylosuccinate synthetase isozyme 2 (ADSS), Adenylosuccinate synthetase isozyme 1 (ADSSL1), Adenylosuccinate synthetase isozyme 2 (ADSS)
  2. Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase, Adenylosuccinate lyase, Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase, Adenylosuccinate lyase (ADSL)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51IMPUniRule annotation1
Binding sitei66IMPUniRule annotation1
Binding sitei130IMPUniRule annotation1
Binding sitei132GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi158 – 160GTPUniRule annotation3
Nucleotide bindingi240 – 242GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processPurine biosynthesisUniRule annotation
LigandGTP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134974111.

Expressioni

Gene expression databases

BgeeiENSG00000185100.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9606.ENSP00000333019.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 132Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
HOGENOMiHOG000260959.
HOVERGENiHBG086952.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiView protein in InterPro
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiView protein in Pfam
PF00709. Adenylsucc_synt. 1 hit.
SMARTiView protein in SMART
SM00788. Adenylsucc_synt. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

B3KTV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPTLEIDIE GQLKRLKGFA ERIRPMVRDG VYFMYEALHG PPKKILVEGA
60 70 80 90 100
NAALLDIDFG TYPFVTSSNC TVGGVCTGLG IPPQNIGDVY GVVKAYTTRV
110 120 130 140 150
GIGAFPTEQI NEIGGLLQTR GHEWGVTTGR KRRCGWLDLM ILRYAHMVNG
160 170 180 190 200
FTALALTKLD ILDVLGEVKV GVSYKLNGKR IPYFPANQEM LQKVEVEYET
210 220 230 240 250
LPGWKADTTG ARRWEDLPPQ AQNYIRFVEN HVGVAVKWVG VGKSRESMIQ

LF
Length:252
Mass (Da):28,079
Last modified:September 2, 2008 - v1
Checksum:iFF8A9B0C036D24B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK096124 mRNA. Translation: BAG53216.1.
AK096720 mRNA. Translation: BAG53356.1.
RefSeqiNP_001307353.1. NM_001320424.1.
NP_954634.1. NM_199165.2.
UniGeneiHs.592327.

Genome annotation databases

GeneIDi122622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK096124 mRNA. Translation: BAG53216.1.
AK096720 mRNA. Translation: BAG53356.1.
RefSeqiNP_001307353.1. NM_001320424.1.
NP_954634.1. NM_199165.2.
UniGeneiHs.592327.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000333019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi122622.

Organism-specific databases

CTDi122622.
PharmGKBiPA134974111.

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
HOGENOMiHOG000260959.
HOVERGENiHBG086952.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Miscellaneous databases

GenomeRNAii122622.

Gene expression databases

BgeeiENSG00000185100.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiView protein in InterPro
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiView protein in Pfam
PF00709. Adenylsucc_synt. 1 hit.
SMARTiView protein in SMART
SM00788. Adenylsucc_synt. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3KTV4_HUMAN
AccessioniPrimary (citable) accession number: B3KTV4
Entry historyiIntegrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: May 10, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.