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B3IUA8

- B3IUA8_SACPS

UniProt

B3IUA8 - B3IUA8_SACPS

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Protein
Submitted name:

Flocculin

Gene
Lg-FLO1
Organism
Saccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi133 – 1331CalciumImported
Metal bindingi134 – 1341CalciumImported
Metal bindingi197 – 1971CalciumImported
Metal bindingi199 – 1991Calcium; via carbonyl oxygenImported
Metal bindingi201 – 2011Calcium; via carbonyl oxygenImported

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. flocculation Source: InterPro
Complete GO annotation...

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
FlocculinImported
Gene namesi
Name:Lg-FLO1Imported
OrganismiSaccharomyces pastorianus (Lager yeast) (Saccharomyces cerevisiae x Saccharomyces eubayanus)Imported
Taxonomic identifieri27292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Imported
Glycosylationi235 – 2351N-linked (GlcNAc...)Imported

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GQ7X-ray2.53A26-245[»]
ProteinModelPortaliB3IUA8.

Family & Domainsi

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamiPF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3IUA8-1 [UniParc]FASTAAdd to Basket

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MTIAHHCIFL VILAFLELLN VASGSTQACL PVGSRKNGMN VNFYKYSLQD     50
STTYSDPQYM AYKYSDTKKL GSVSGQTHLS IYYGPNTAFW NTASWSSDLF 100
GFYTTPTNVT VEMTGYFLPP QTGSYTFKFA TVDDSAILSV GGSIAFECCA 150
QEQPPITSTD FTINGIKPWD AATPTDIKGS TYMYAGYYYP IKIVYSNAKA 200
LARLPVSVVL PDGTEVNDDF EGYVYSFDDD LSQSNCTIPD PSKHTTSIVT 250
TTTELWTGTF TSTSTEMTTI TGTNGVPTDE TIIVVKTPTI ASTAITTTEP 300
WTGTFTSTST EMTTVTGTNG LPTDETIIVI RTPTTASTAI TTTEPWTGTF 350
TSTSTEMTTV TGTNGLPTDE TIIVVKTPTI ASTAITTTEP WTGTSTSTST 400
ELTTVTGTNG LPTDETIIVV RTPTTASTII TTTEPWTGTF TSTSTEMTTV 450
TGTNGQPTDE TVIVVKTPTT ANTIVTTTKP WTGTFTSTST EMTTVTGTNG 500
QPTDETVIVI RTPTSEGLIS TTTEPWTGTF TSTSTEVTTI TGTNGQPTDE 550
TVIVIRTPTS EGLVTTTTEP WTGTFTSTST GMTTITGTNG LPTDETVIVV 600
KTPTTAISSS LSLSSSGQIT SSITSSRPII TPFYPSNGTS VISSSDTSSS 650
DTSSLVTSSL VTSSLVTSSV ISSSVTSSLV TSSVISSSVT SSLVTSSVIS 700
SSVTSSLFTS SPVISSSVIS SSTTTSTSIF FESSKSSVIP TSSSTSGSSE 750
SKTSSASSSS SSSSISSESP KSTYSSSSLP PVTSATTSQE TASSLPPVTS 800
VTTSQEITSS LPPVISTTTS QETASSLPPV TSATTSQETA SSLPPVTSVT 850
TSQEITSSLP PVTSVTTSQE ITSSLPPVTS ATTSQEITSS LPPVTSATTS 900
QETASSLPPV TSAATSQETA SSLPPVTSVT TSQEITSSLP PVTSVTTSQE 950
ITSSLPPVTS VTTSQEITSS LPPVTSATTS QETASSLPPA TTTKTSEQTT 1000
LVTVTSCESH VCTESISSAI VSTATVTVSG VTTEYTTWCP ISTTEITKQT 1050
TETTKQTKGT TKQTKGTTEQ TTETTKQTTV VTISSCESDI CSKTASPAIV 1100
STSTATINDV TTEYTTWCPI STTESKQQTT LVTVTSCESG VCSETASPAI 1150
VSTATATVND VVTVYPTWRP QTTNEQSVSS KMNSATSETT TNTGAAETKT 1200
AVTSSLSRFN HAETQTASAT DVIGHSSSVV SVSETGNTKG LITSELSTMS 1250
QQPRSTPAST MVGSSTASLE ISTYVGIANG LLTNNGISVF ISTVLLAIVW 1300
Length:1,300
Mass (Da):134,697
Last modified:September 2, 2008 - v1
Checksum:i3345C5106C434DA0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB288349 Genomic DNA. Translation: BAG49462.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB288349 Genomic DNA. Translation: BAG49462.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GQ7 X-ray 2.53 A 26-245 [» ]
ProteinModelPortali B3IUA8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.182.10. 1 hit.
InterProi IPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view ]
Pfami PF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view ]
SMARTi SM00758. PA14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chromosomal location of Lg-FLO1 in bottom-fermenting yeast and the FLO5 locus of industrial yeast."
    Ogata T., Izumikawa M., Kohno K., Shibata K.
    J. Appl. Microbiol. 105:1186-1198(2008)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: FY-2Imported.
  2. "Structural and biochemical characterization of the N-terminal domain of flocculin Lg-Flo1p from Saccharomyces pastorianus reveals a unique specificity for phosphorylated mannose."
    Sim L., Groes M., Olesen K., Henriksen A.
    FEBS J. 280:1073-1083(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS) OF 26-245 IN COMPLEX WITH CALCIUM, GLYCOSYLATION AT ASN-108 AND ASN-235.

Entry informationi

Entry nameiB3IUA8_SACPS
AccessioniPrimary (citable) accession number: B3IUA8
Entry historyi
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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