Reviewed, UniProtKB/Swiss-Prot B3H307 (HEMH_ACTP7)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	APP7_2025

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ferrochelatase HAMAP MF_00323		PRO_1000116026
Sites
	Metal binding	193	1	Iron By similarity
	Metal binding	274	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H307-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 25C77B9A4772B262	FASTA	319	36,253
	10 20 30 40 50 60 MNTNKIGVLL ANLGTPDEPT TPAVKRYLKQ FLSDPRVIDL PKFKWQFILN YMILPKRSPK 70 80 90 100 110 120 VAKLYREIWT EQGSPLLAIS RQQQQALQDY FNRQNQNVLV ELGMSYGNPS IESATDRLIK 130 140 150 160 170 180 AGVSKIIVLP LYPQYSSTTT ASVLDAFARG LTQQRNIVPF EFIHSYHNDP LYIQALANTI 190 200 210 220 230 240 QLAEDEKLLF SFHGIPKRYQ TEGDFYPEHC QQTAQLVADK LSLTDEQWLV TYQSRFGDEE 250 260 270 280 290 300 WLQPYTDETL ETLPSQGVKK IAVICAGFSA DCLETLEEIA EENKENFLNA GGQSYRYIPA 310 LNANTDHINA LAKLIEAKI

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE62677.1.
RefSeq	YP_001969819.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6397871.
GenomeReviews	Gene locus APP7_2025 in contig CP001091_GR.
KEGG	apa:APP7_2025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_ACTP7

Accession

Primary (citable) accession number: B3H307

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)