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B3H2S8

- B3H2S8_ACTP7

UniProt

B3H2S8 - B3H2S8_ACTP7

Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei679 – 6791ATP; via carbonyl oxygenUniRule annotation
    Metal bindingi680 – 6801MagnesiumUniRule annotation
    Metal bindingi719 – 7191MagnesiumUniRule annotation
    Metal bindingi723 – 7231MagnesiumUniRule annotation
    Metal bindingi887 – 8871MagnesiumUniRule annotation
    Binding sitei889 – 8891ATPUniRule annotation
    Active sitei1138 – 11381NucleophileUniRule annotation
    Active sitei1263 – 12631UniRule annotation
    Active sitei1265 – 12651UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi308 – 31912ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Purine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAPLE537457:GJI0-1807-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Protein family/group databases

    MEROPSiC56.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Short name:
    FGAMSUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
    Gene namesi
    Name:purLUniRule annotationImported
    Ordered Locus Names:APP7_1743Imported
    OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)Imported
    Taxonomic identifieri537457 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001226: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi537457.APP7_1743.

    Structurei

    3D structure databases

    ProteinModelPortaliB3H2S8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
    Contains glutamine amidotransferase type-1 domain.SAAS annotation
    In the N-terminal section; belongs to the FGAMS family.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferaseUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG0046.
    HOGENOMiHOG000261359.
    KOiK01952.
    OMAiGEGHAEF.
    OrthoDBiEOG6FNHHR.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00419. PurL_1.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3H2S8-1 [UniParc]FASTAAdd to Basket

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    MTVQTFRGSP ALSEFRLNQF QTKFQQNALP VKSVYAEYVH FVDLNAELSA     50
    AQTAEIKELL HYGPTLAEHE PVGFCLIVTP RIGTISSWSS KATDIAHNCG 100
    LQAVNRIERG LAYYFEFSAE PTAAQIETLK GLLHDRMLET VLDNEAQAAN 150
    LFAQQEPKPF TTVDILNGGR KALEEANVNL GLALADDEID YLVENFTALK 200
    RNPNDIELYM FAQANSEHCR HKIFNADWTI DGQKQEKSLF KMIKNTFEKT 250
    PDYVLSAYKD NAAVMEGSKV GRWFPDQDGQ YRAHQEDAHI LMKVETHNHP 300
    TAISPFPGAA TGSGGEIRDE GATGRGAKPK AGLTGFSVSN LVIPNFEQPW 350
    ENPLSKPHRI ASALDIMIEG PLGGAAFNNE FGRPALLGYF RTYEEKVNSF 400
    AGEEVRGYHK PIMLAGGIGN IRAEHVQKGE IPVGAKLIVL GGPAMNIGLG 450
    GGAASSMASG KSKEDLDFAS VQRENPEMER RCQEVIDRCW QLGEDNPILF 500
    IHDVGAGGLS NAMPELVHDG DRGGKFDLRK ILCDEKGMSP LEIWCNESQE 550
    RYVLAVSPEK LALFTELCER ERAPFAVIGE ATEEKHLTLK DDHFDNNPID 600
    LPMNVLLGKT PKMHRDVSSK TVENQPLDSQ QIQLKEALHR VLRLPVVAEK 650
    TFLITIGDRT VTGMVARDQM VGPWQIPVAD CAVTTASLDS YHGEAMSMGE 700
    RAPVALLDFG ASARLAVAES ITNIAATNIG ELKRIKLSAN WMSAAGHEGE 750
    DAGLYEAVKA VGEELCPALG ITIPVGKDSM SMRTTWEENG EQKSVTAPLS 800
    LVISAFARVE DVRKTVTPQL RTDKGLSSLL LLDLGEGKNR LGATALAQVY 850
    KQLGDKPADV VNVESLKNFF NAMQTLVAED KLLAYHDRSD GGLITTLAEM 900
    TFAGNCGVEI DISALGDDDL AVLFNEELGA VIQVKDADLA RVREVLNAHN 950
    LLGLTKDLGT VHEDDRFVIS RGSLKLLNEK RSELRGIWAE LTHQMQRLRD 1000
    NPECADQEFE TKKDPNNKGL STFLTYDVNE DITAPFINKG VKPTIAILRE 1050
    QGVNSHYEMA AAFDRAGFNA IDVHMSDLMA GRRNLKDFNA LVACGGFSYG 1100
    DVLGAGGGWA KSILFNPMLR EQFSQFFANP NTLALGVCNG CQMVSNLAEI 1150
    IPGTENWPHF VRNKSERFEA RVGLVKINET NSHWFQGMAG SHMPIAVSHG 1200
    EGQVEFKSAQ QLADLTAQKL VIAQYIDNNL QPTEVYPANP NGSVNGITAI 1250
    SNSDGRVAIM MPHPERVYRA VSNSWHPEDW TEDGAWMRLF RNARVVLG 1298
    Length:1,298
    Mass (Da):142,534
    Last modified:July 22, 2008 - v1
    Checksum:iC52E931B0B9C035A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001091 Genomic DNA. Translation: ACE62395.1.
    RefSeqiYP_001969537.1. NC_010939.1.

    Genome annotation databases

    EnsemblBacteriaiACE62395; ACE62395; APP7_1743.
    GeneIDi6396573.
    KEGGiapa:APP7_1743.
    PATRICi20757258. VBIActPle116979_1785.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001091 Genomic DNA. Translation: ACE62395.1 .
    RefSeqi YP_001969537.1. NC_010939.1.

    3D structure databases

    ProteinModelPortali B3H2S8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 537457.APP7_1743.

    Protein family/group databases

    MEROPSi C56.972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE62395 ; ACE62395 ; APP7_1743 .
    GeneIDi 6396573.
    KEGGi apa:APP7_1743.
    PATRICi 20757258. VBIActPle116979_1785.

    Phylogenomic databases

    eggNOGi COG0046.
    HOGENOMi HOG000261359.
    KOi K01952.
    OMAi GEGHAEF.
    OrthoDBi EOG6FNHHR.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci APLE537457:GJI0-1807-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00419. PurL_1.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
      Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AP76Imported.

    Entry informationi

    Entry nameiB3H2S8_ACTP7
    AccessioniPrimary (citable) accession number: B3H2S8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 22, 2008
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3