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B3H2S8

- B3H2S8_ACTP7

UniProt

B3H2S8 - B3H2S8_ACTP7

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Protein

Phosphoribosylformylglycinamidine synthase

Gene
purL, APP7_1743
Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate By similarity.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATP; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi680 – 6801Magnesium By similarityUniRule annotation
Metal bindingi719 – 7191Magnesium By similarityUniRule annotation
Metal bindingi723 – 7231Magnesium By similarityUniRule annotation
Metal bindingi887 – 8871Magnesium By similarityUniRule annotation
Binding sitei889 – 8891ATP By similarityUniRule annotation
Active sitei1138 – 11381Nucleophile By similarityUniRule annotation
Active sitei1263 – 12631 By similarityUniRule annotation
Active sitei1265 – 12651 By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi308 – 31912ATP By similarityUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotationsImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-1807-MONOMER.
UniPathwayiUPA00074; UER00128.

Protein family/group databases

MEROPSiC56.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Gene namesi
Name:purLUniRule annotationImported
Ordered Locus Names:APP7_1743Imported
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)Imported
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001226: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi537457.APP7_1743.

Structurei

3D structure databases

ProteinModelPortaliB3H2S8.

Family & Domainsi

Sequence similaritiesi

Contains glutamine amidotransferase type-1 domain.SAAS annotations
Contains glutamine amidotransferase type-domain.SAAS annotations
In the N-terminal section; belongs to the FGAMS family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotations

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000261359.
KOiK01952.
OMAiGEGHAEF.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3H2S8-1 [UniParc]FASTAAdd to Basket

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MTVQTFRGSP ALSEFRLNQF QTKFQQNALP VKSVYAEYVH FVDLNAELSA     50
AQTAEIKELL HYGPTLAEHE PVGFCLIVTP RIGTISSWSS KATDIAHNCG 100
LQAVNRIERG LAYYFEFSAE PTAAQIETLK GLLHDRMLET VLDNEAQAAN 150
LFAQQEPKPF TTVDILNGGR KALEEANVNL GLALADDEID YLVENFTALK 200
RNPNDIELYM FAQANSEHCR HKIFNADWTI DGQKQEKSLF KMIKNTFEKT 250
PDYVLSAYKD NAAVMEGSKV GRWFPDQDGQ YRAHQEDAHI LMKVETHNHP 300
TAISPFPGAA TGSGGEIRDE GATGRGAKPK AGLTGFSVSN LVIPNFEQPW 350
ENPLSKPHRI ASALDIMIEG PLGGAAFNNE FGRPALLGYF RTYEEKVNSF 400
AGEEVRGYHK PIMLAGGIGN IRAEHVQKGE IPVGAKLIVL GGPAMNIGLG 450
GGAASSMASG KSKEDLDFAS VQRENPEMER RCQEVIDRCW QLGEDNPILF 500
IHDVGAGGLS NAMPELVHDG DRGGKFDLRK ILCDEKGMSP LEIWCNESQE 550
RYVLAVSPEK LALFTELCER ERAPFAVIGE ATEEKHLTLK DDHFDNNPID 600
LPMNVLLGKT PKMHRDVSSK TVENQPLDSQ QIQLKEALHR VLRLPVVAEK 650
TFLITIGDRT VTGMVARDQM VGPWQIPVAD CAVTTASLDS YHGEAMSMGE 700
RAPVALLDFG ASARLAVAES ITNIAATNIG ELKRIKLSAN WMSAAGHEGE 750
DAGLYEAVKA VGEELCPALG ITIPVGKDSM SMRTTWEENG EQKSVTAPLS 800
LVISAFARVE DVRKTVTPQL RTDKGLSSLL LLDLGEGKNR LGATALAQVY 850
KQLGDKPADV VNVESLKNFF NAMQTLVAED KLLAYHDRSD GGLITTLAEM 900
TFAGNCGVEI DISALGDDDL AVLFNEELGA VIQVKDADLA RVREVLNAHN 950
LLGLTKDLGT VHEDDRFVIS RGSLKLLNEK RSELRGIWAE LTHQMQRLRD 1000
NPECADQEFE TKKDPNNKGL STFLTYDVNE DITAPFINKG VKPTIAILRE 1050
QGVNSHYEMA AAFDRAGFNA IDVHMSDLMA GRRNLKDFNA LVACGGFSYG 1100
DVLGAGGGWA KSILFNPMLR EQFSQFFANP NTLALGVCNG CQMVSNLAEI 1150
IPGTENWPHF VRNKSERFEA RVGLVKINET NSHWFQGMAG SHMPIAVSHG 1200
EGQVEFKSAQ QLADLTAQKL VIAQYIDNNL QPTEVYPANP NGSVNGITAI 1250
SNSDGRVAIM MPHPERVYRA VSNSWHPEDW TEDGAWMRLF RNARVVLG 1298
Length:1,298
Mass (Da):142,534
Last modified:July 22, 2008 - v1
Checksum:iC52E931B0B9C035A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE62395.1.
RefSeqiYP_001969537.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE62395; ACE62395; APP7_1743.
GeneIDi6396573.
KEGGiapa:APP7_1743.
PATRICi20757258. VBIActPle116979_1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE62395.1 .
RefSeqi YP_001969537.1. NC_010939.1.

3D structure databases

ProteinModelPortali B3H2S8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 537457.APP7_1743.

Protein family/group databases

MEROPSi C56.972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE62395 ; ACE62395 ; APP7_1743 .
GeneIDi 6396573.
KEGGi apa:APP7_1743.
PATRICi 20757258. VBIActPle116979_1785.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000261359.
KOi K01952.
OMAi GEGHAEF.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci APLE537457:GJI0-1807-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00419. PurL_1.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
    Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AP76.

Entry informationi

Entry nameiB3H2S8_ACTP7
AccessioniPrimary (citable) accession number: B3H2S8
Entry historyi
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: September 3, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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