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B3H2S8

- B3H2S8_ACTP7

UniProt

B3H2S8 - B3H2S8_ACTP7

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Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATP; via carbonyl oxygenUniRule annotation
Metal bindingi680 – 6801MagnesiumUniRule annotation
Metal bindingi719 – 7191MagnesiumUniRule annotation
Metal bindingi723 – 7231MagnesiumUniRule annotation
Metal bindingi887 – 8871MagnesiumUniRule annotation
Binding sitei889 – 8891ATPUniRule annotation
Active sitei1138 – 11381NucleophileUniRule annotation
Active sitei1263 – 12631UniRule annotation
Active sitei1265 – 12651UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi308 – 31912ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-1807-MONOMER.
UniPathwayiUPA00074; UER00128.

Protein family/group databases

MEROPSiC56.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotationSAAS annotation (EC:6.3.5.3UniRule annotationSAAS annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
Gene namesi
Name:purLUniRule annotationImported
Ordered Locus Names:APP7_1743Imported
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)Imported
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001226: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi537457.APP7_1743.

Structurei

3D structure databases

ProteinModelPortaliB3H2S8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
Contains glutamine amidotransferase type-1 domain.SAAS annotation
Contains glutamine amidotransferase type-domain.SAAS annotation
In the N-terminal section; belongs to the FGAMS family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000261359.
KOiK01952.
OMAiGEGHAEF.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3H2S8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVQTFRGSP ALSEFRLNQF QTKFQQNALP VKSVYAEYVH FVDLNAELSA
60 70 80 90 100
AQTAEIKELL HYGPTLAEHE PVGFCLIVTP RIGTISSWSS KATDIAHNCG
110 120 130 140 150
LQAVNRIERG LAYYFEFSAE PTAAQIETLK GLLHDRMLET VLDNEAQAAN
160 170 180 190 200
LFAQQEPKPF TTVDILNGGR KALEEANVNL GLALADDEID YLVENFTALK
210 220 230 240 250
RNPNDIELYM FAQANSEHCR HKIFNADWTI DGQKQEKSLF KMIKNTFEKT
260 270 280 290 300
PDYVLSAYKD NAAVMEGSKV GRWFPDQDGQ YRAHQEDAHI LMKVETHNHP
310 320 330 340 350
TAISPFPGAA TGSGGEIRDE GATGRGAKPK AGLTGFSVSN LVIPNFEQPW
360 370 380 390 400
ENPLSKPHRI ASALDIMIEG PLGGAAFNNE FGRPALLGYF RTYEEKVNSF
410 420 430 440 450
AGEEVRGYHK PIMLAGGIGN IRAEHVQKGE IPVGAKLIVL GGPAMNIGLG
460 470 480 490 500
GGAASSMASG KSKEDLDFAS VQRENPEMER RCQEVIDRCW QLGEDNPILF
510 520 530 540 550
IHDVGAGGLS NAMPELVHDG DRGGKFDLRK ILCDEKGMSP LEIWCNESQE
560 570 580 590 600
RYVLAVSPEK LALFTELCER ERAPFAVIGE ATEEKHLTLK DDHFDNNPID
610 620 630 640 650
LPMNVLLGKT PKMHRDVSSK TVENQPLDSQ QIQLKEALHR VLRLPVVAEK
660 670 680 690 700
TFLITIGDRT VTGMVARDQM VGPWQIPVAD CAVTTASLDS YHGEAMSMGE
710 720 730 740 750
RAPVALLDFG ASARLAVAES ITNIAATNIG ELKRIKLSAN WMSAAGHEGE
760 770 780 790 800
DAGLYEAVKA VGEELCPALG ITIPVGKDSM SMRTTWEENG EQKSVTAPLS
810 820 830 840 850
LVISAFARVE DVRKTVTPQL RTDKGLSSLL LLDLGEGKNR LGATALAQVY
860 870 880 890 900
KQLGDKPADV VNVESLKNFF NAMQTLVAED KLLAYHDRSD GGLITTLAEM
910 920 930 940 950
TFAGNCGVEI DISALGDDDL AVLFNEELGA VIQVKDADLA RVREVLNAHN
960 970 980 990 1000
LLGLTKDLGT VHEDDRFVIS RGSLKLLNEK RSELRGIWAE LTHQMQRLRD
1010 1020 1030 1040 1050
NPECADQEFE TKKDPNNKGL STFLTYDVNE DITAPFINKG VKPTIAILRE
1060 1070 1080 1090 1100
QGVNSHYEMA AAFDRAGFNA IDVHMSDLMA GRRNLKDFNA LVACGGFSYG
1110 1120 1130 1140 1150
DVLGAGGGWA KSILFNPMLR EQFSQFFANP NTLALGVCNG CQMVSNLAEI
1160 1170 1180 1190 1200
IPGTENWPHF VRNKSERFEA RVGLVKINET NSHWFQGMAG SHMPIAVSHG
1210 1220 1230 1240 1250
EGQVEFKSAQ QLADLTAQKL VIAQYIDNNL QPTEVYPANP NGSVNGITAI
1260 1270 1280 1290
SNSDGRVAIM MPHPERVYRA VSNSWHPEDW TEDGAWMRLF RNARVVLG
Length:1,298
Mass (Da):142,534
Last modified:July 22, 2008 - v1
Checksum:iC52E931B0B9C035A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE62395.1.
RefSeqiYP_001969537.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE62395; ACE62395; APP7_1743.
GeneIDi6396573.
KEGGiapa:APP7_1743.
PATRICi20757258. VBIActPle116979_1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE62395.1 .
RefSeqi YP_001969537.1. NC_010939.1.

3D structure databases

ProteinModelPortali B3H2S8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 537457.APP7_1743.

Protein family/group databases

MEROPSi C56.972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE62395 ; ACE62395 ; APP7_1743 .
GeneIDi 6396573.
KEGGi apa:APP7_1743.
PATRICi 20757258. VBIActPle116979_1785.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000261359.
KOi K01952.
OMAi GEGHAEF.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci APLE537457:GJI0-1807-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00419. PurL_1.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
    Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AP76Imported.

Entry informationi

Entry nameiB3H2S8_ACTP7
AccessioniPrimary (citable) accession number: B3H2S8
Entry historyi
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3