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B3H2D1

- NAPA_ACTP7

UniProt

B3H2D1 - NAPA_ACTP7

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Protein
Periplasmic nitrate reductase
Gene
napA, APP7_1504
Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S) By similarity
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-1549-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:APP7_1504
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001226: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signal Reviewed prediction
Add
BLAST
Chaini33 – 827795Periplasmic nitrate reductaseUniRule annotation
PRO_1000186348Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi537457.APP7_1504.

Structurei

3D structure databases

ProteinModelPortaliB3H2D1.
SMRiB3H2D1. Positions 35-826.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3H2D1-1 [UniParc]FASTAAdd to Basket

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MELNRRDFMK ANAAMAAAAA AGMTIPVKNV YAADDGIRWD KAPCRFCGTG    50
CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL 100
RMKDGKFHKE GDFTPVSWDQ AFTIMAEKVK DILKKKEPNS VGMFSSGQTT 150
IFEGYAKVKL WKGGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND 200
IEKTDAFVLW GSNMAEMHPI LWSRISDRRL SDDKVKVVVM STFEHRSFEL 250
ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 300
LRPDHPRQKA AKNAKTAGKM YDSDFEEFKK IVEPYTLEKA HEISGVPKDQ 350
LETLAKMYAD PKQTLVSFWT MGFNQHVRGV WVNHMVYNVH LLTGKISTPG 400
CGPFSLTGQP SACGTAREVG TFVHRLPADM VVTNPKHVEI VEKAWKLPKG 450
TIPTVPGYPA VMQSRMLKDG KLNFLWQLCT NNMQGGPNIN EEIFPGWRNP 500
ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR TQFWRQQVKG 550
PGESKSDLWQ IVEFSKYFKT DEVWDEALLA QMPEYRGKTL YEVLYKNGQV 600
DKFDTPTNIP GYINDEAEHF GYYLQKGLFE EYAAFGRGHG HDLADFDTYH 650
QVRGLRWPVV DGKETLWRYR EGFDPYVKAG EGVSFYGYPD KKAIILGVPY 700
EEPAESPDEE YDLWLCTGRV LEHWHTGTMT RRVPELHRSF PNNLCWMHPD 750
DAKARGLRHG DKVKLITRRG EIITHLDTRG RNKCPKGLIY TTFFDAGQLA 800
NKLTLDATDP ISGETDYKKC AVKVVKA 827
Length:827
Mass (Da):93,572
Last modified:July 22, 2008 - v1
Checksum:iBE020AD5642AF456
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE62156.1.
RefSeqiYP_001969298.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE62156; ACE62156; APP7_1504.
GeneIDi6397518.
KEGGiapa:APP7_1504.
PATRICi20756720. VBIActPle116979_1537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE62156.1 .
RefSeqi YP_001969298.1. NC_010939.1.

3D structure databases

ProteinModelPortali B3H2D1.
SMRi B3H2D1. Positions 35-826.
ModBasei Search...

Protein-protein interaction databases

STRINGi 537457.APP7_1504.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE62156 ; ACE62156 ; APP7_1504 .
GeneIDi 6397518.
KEGGi apa:APP7_1504.
PATRICi 20756720. VBIActPle116979_1537.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci APLE537457:GJI0-1549-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
    Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AP76.

Entry informationi

Entry nameiNAPA_ACTP7
AccessioniPrimary (citable) accession number: B3H2D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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