Reviewed, UniProtKB/Swiss-Prot B3H269 (MDH_ACTP7)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	APP7_1347

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01516
Subunit structure	Homodimer By similarity. HAMAP MF_01516
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Malate dehydrogenase HAMAP MF_01516		PRO_1000146165
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	231	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H269-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 0108DFF7EDA51B35	FASTA	317	33,405
	10 20 30 40 50 60 MKVALLGAAG GIGQTLALLL KLRLPVGTDL ALYDISPVTP GIAVDISHIP TSVSAVGYSG 70 80 90 100 110 120 EDPSEALKGA NLVIITAGVA RKPGMTRADL FNINADIVKN LVEKVAEVCP KACIGIVTNP 130 140 150 160 170 180 VNTLVPIAAE VLRKAGVYDK RKLFGVTTLD VVRAKTFTSE LKEKHVETVK VPVIGGHSGP 190 200 210 220 230 240 TILPLLSQAL SEGLPLSFTQ EEIEALTYRI QNAGTEVVEA KAGGGSATLS MAESGARFAV 250 260 270 280 290 300 AVFKALLGED CVRYAYVESK EGSGYPEFFA HPVRFGLTGV EELLPIGKLS EYEQAKLDEL 310 KPVLEADIAL GKNFVNP

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61999.1.
RefSeq	YP_001969141.1.
3D structure databases
SMR	B3H269. Positions 1-316.
ModBase	Search...
Genome annotation databases
GeneID	6396854.
GenomeReviews	Gene locus APP7_1347 in contig CP001091_GR.
KEGG	apa:APP7_1347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	RDAMLDT.
Family and domain databases
HAMAP	MF_01516. Malate_dehydrog_1. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACTP7

Accession

Primary (citable) accession number: B3H269

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)