B3H248 (SYE_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	APP7_1326

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 7

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_1000090049
Regions
	Motif	21 – 31	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	248 – 252	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H248 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 7198581463E9728C	FASTA	479	54,293
	10 20 30 40 50 60 MNIETLFPLD PNVKVRTRFA PSPTGYLHVG GARTALYSWL YAKHFNGEFV LRIEDTDLER 70 80 90 100 110 120 STPEATAAIL EGMEWLNLAW EHGPYYQTKR FDRYNQVIDQ MIEQGLAYRC YCSKERLENL 130 140 150 160 170 180 RHEQEANKEK PRYDRHCLAH HDQPTDAPHV VRFKNPQEGS VVFDDAVRGR IEISNSELDD 190 200 210 220 230 240 LIIRRTDGSP TYNFCVVVDD WDMGITHVVR GEDHINNTPR QINILKALGA PIPTYAHVSM 250 260 270 280 290 300 INGDDGQKLS KRHGAVSVMQ YRDDGYLPEA LINYLVRLGW GHGDQEIFSR EEMIELFDIH 310 320 330 340 350 360 SVSKSASAFN TDKLQWLNQH YMRSLPAEHV AKYLAWHMND QAIDTSNGPA LEEIIPVLSE 370 380 390 400 410 420 RAKTLKELAA ASRYFYQEFD GYDEKAAAKN FKAEAVAPLA KLLEKLTALT DWSVEAIHDA 430 440 450 460 470 MNATAADLEI GMGKVGMPFR LAVTGSGQSP SMDITAKLVG RERTLARIQK AIEFIQAQA

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61978.1.
RefSeq	YP_001969120.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3H248.
ModBase	Search...
Protein-protein interaction databases
STRING	537457.APP7_1326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE61978; ACE61978; APP7_1326.
GeneID	6396648.
KEGG	apa:APP7_1326.
PATRIC	20756323. VBIActPle116979_1355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	RYRDFKG.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	APLE537457:GJI0-1356-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_ACTP7

Accession

Primary (citable) accession number: B3H248

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program