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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-951-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:APP7_0931
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001226 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834Leucine--tRNA ligasePRO_1000091282Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB3H1L1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3H1L1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQQYNPSAI EPKVQQFWAE NKVFKAVKDV TKEKYYCLSM LPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VVSRYQRMIG KNVLQPMGWD AFGLPAEGAA VKNNTAPAKW
110 120 130 140 150
TYENIEYMKG QLKMLGFSYD WDREVTTCRQ EYYKWEQWFF TELYKKGLVY
160 170 180 190 200
KKTSTVNWCP NDATVLANEQ VHEGCCWRCD TPVEQREIPQ WFIKITDYAE
210 220 230 240 250
ELLTHLDNLP QWPDQVKTMQ RNWIGRSEGV EITFKIAGSN AELPVYTTRP
260 270 280 290 300
DTFFGVSYVA IAAAHPLAEM AAENNPALAE FIREAKNTKV AEAELATMEK
310 320 330 340 350
KGMATGLFAI HPLTGKEVPV WVANFVLMHY GTGAVMAVPA HDERDFEFAQ
360 370 380 390 400
KYGLQINQVI QPLDGSEWDF SKAAYTEHGK LINSAEFDDL NFEQAFNAIA
410 420 430 440 450
DKLESMKVGK RQVNFRLRDW GVSRQRYWGA PIPMMTTEDG EVVTVPMQDL
460 470 480 490 500
PVILPEDVVM NGVQSPIKAD PEWAKTTYNG KPALKETDTF DTFMESSWYY
510 520 530 540 550
ARYTCPQYHE GMLDSDEANY WLPVDQYIGG IEHATMHLLY FRFFHKLLRD
560 570 580 590 600
AGILNSDEPA TKLLCQGMVL ADAFYYTSPT NERIWVSPTQ VTLERDEKGR
610 620 630 640 650
IIKATDPEGR ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS
660 670 680 690 700
PAEMTLEWQE SGVEGAKRFL GRVWNLVYEY SQNPATAALD VAALSKAQKE
710 720 730 740 750
LRRDVHKTIA KVSDDIGRRQ TFNTAIAAIM ELMNKLTKAP LENEQDKAVM
760 770 780 790 800
AEALSAVVRM LYPITPHICF ELWQALGNND TIDFAPWVVA DESAMVEDEK
810 820 830
LVVVQVNGKV RVKLPFRQQQ PKMKSKQLRK RMQT
Length:834
Mass (Da):95,323
Last modified:July 22, 2008 - v1
Checksum:i52784EF8F74B087F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE61583.1.
RefSeqiWP_012478450.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE61583; ACE61583; APP7_0931.
KEGGiapa:APP7_0931.
PATRICi20755498. VBIActPle116979_0953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE61583.1.
RefSeqiWP_012478450.1. NC_010939.1.

3D structure databases

ProteinModelPortaliB3H1L1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE61583; ACE61583; APP7_0931.
KEGGiapa:APP7_0931.
PATRICi20755498. VBIActPle116979_0953.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-951-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
    Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AP76.

Entry informationi

Entry nameiSYL_ACTP7
AccessioniPrimary (citable) accession number: B3H1L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: July 22, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.