Reviewed, UniProtKB/Swiss-Prot B3H1K7 (SYS_ACTP7)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	APP7_0927

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP MF_00176
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity. HAMAP MF_00176
Subcellular location	Cytoplasm By similarity HAMAP MF_00176.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP MF_00176
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Seryl-tRNA synthetase HAMAP MF_00176		PRO_1000098026
Regions
	Nucleotide binding	272 – 274	3	ATP By similarity
	Nucleotide binding	359 – 362	4	ATP By similarity
	Region	241 – 243	3	Serine binding By similarity
Sites
	Binding site	295	1	Serine By similarity
	Binding site	395	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H1K7-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B1123754200356CC	FASTA	435	48,366
	10 20 30 40 50 60 MIDQNLLRTN LDDVANALKV KRGFTLDVES VKALEEKRKA LQVKTETLQA ERNARSKNIG 70 80 90 100 110 120 AAKARGEDIS ALLAEVDNMG NELNEAKVAL DQVQAEIREL LLSVPNLPAD EVPLGKDDTE 130 140 150 160 170 180 NLEVSRWGEP RQFDFEVKDH VALGEALNGL DFAAGVKLTA SRFVVMKGKL ARLHRALSQF 190 200 210 220 230 240 MLDLHTEQHG YVETNVPFLV NHDTLFGTGQ LPKFGEDLFH TQPLTGQDPN ETQRPFSLIP 250 260 270 280 290 300 TAEVPVTNLV RDEIIDEDSL PLRYTAHTPC FRSEAGSYGR DTRGLIRMHQ FEKVEMVQIV 310 320 330 340 350 360 APEKSMEALE ELTGHAEKVL QLLGLPYRKV LLCTGDMGFG SAKTYDLEVW LPAQNTYREI 370 380 390 400 410 420 SSCSNMWDFQ ARRMSARCKA KGDKKTRLVH TLNGSGLAVG RTLVAVLENY QNADGSITVP 430 EVLRPYMGGV EVITA

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61579.1.
RefSeq	YP_001968721.1.
3D structure databases
SMR	B3H1K7. Positions 1-434.
ModBase	Search...
Genome annotation databases
GeneID	6395882.
GenomeReviews	Gene locus APP7_0927 in contig CP001091_GR.
KEGG	apa:APP7_0927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629391.
OMA	AHWDLGP.
Family and domain databases
HAMAP	MF_00176. Ser_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA_bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_ACTP7

Accession

Primary (citable) accession number: B3H1K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)