B3H1B7 (ISPE_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	APP7_0837

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092056
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H1B7 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 3E1D3DE09808845E	FASTA	285	31,396
	10 20 30 40 50 60 MTKKIILPSP AKLNLFLYIT NKRVDGYHEL QTLFQFLDFG DDISLEVNES GEIELLNAIE 70 80 90 100 110 120 GVAKEQNLIY RAAKLLQNHT ACSKGAKIGV TKRLPMGGGV GGGSSNAATV LVGLNHFWQT 130 140 150 160 170 180 GLSLEQLAEL GLSLGADVPI FVRGFAAFAE GVGEKLVACQ PRESWYVVLK PNVSISTAAV 190 200 210 220 230 240 FQDPNLPRNT PKRTLSRLLS EEWTNDCEKV VRDHYFEVED LIAELLQYAT FRLTGTGACI 250 260 270 280 FAEFESEAEA KAVFAHKPHN IFGFIAKGQN RSPLHQMLNL TTFPQ

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61489.1.
RefSeq	YP_001968631.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3H1B7.
ModBase	Search...
Protein-protein interaction databases
STRING	B3H1B7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6396016.
GenomeReviews	Gene locus APP7_0837 in contig CP001091_GR.
KEGG	apa:APP7_0837.
PATRIC	20755301. VBIActPle116979_0857.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ACTP7

Accession

Primary (citable) accession number: B3H1B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program