Reviewed, UniProtKB/Swiss-Prot B3H1B5 (PYRD_ACTP7)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	APP7_0835

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000100244
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H1B5-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 72EC7D5CFCD54DB2	FASTA	335	36,197
	10 20 30 40 50 60 MYSLIRKCLF SMDAETAHNF SIQALKLAGK LPINVLPMPL NPVEVMGLQF KNPIGLAAGA 70 80 90 100 110 120 DKNGEAIDGF GKLGFGFIEV GTVTPVAQDG NPKPRQFRIL EAEGIINRNG FNNLGVDVLV 130 140 150 160 170 180 ENVKKAKYDG IIGINIGKNA VTPIERALDD YQICLRKVYE HADYITVNIS SPNTKNLRTL 190 200 210 220 230 240 QYGEALDDLL RSLKSEQESL SQKFNRYKPL VLKIAPDLTD EEIASVADGL IRHKIDGVIA 250 260 270 280 290 300 GNTTLSRDPV VGLKNAEQQG GLSGKPLNTL STRLISTLAE ELNGTLPIIG SGGIHSVASG 310 320 330 QEKIDAGASL LQVYSAMIYQ GLALIQNLAK HIQVR

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61487.1.
RefSeq	YP_001968629.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396020.
GenomeReviews	Gene locus APP7_0835 in contig CP001091_GR.
KEGG	apa:APP7_0835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NAEQQGG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACTP7

Accession

Primary (citable) accession number: B3H1B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)