B3H137 (SYFA_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	APP7_0654

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000114842
Sites
	Metal binding	253	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H137 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: C2EDBDF105658A5A	FASTA	328	37,611
	10 20 30 40 50 60 MQHLKELTEK ARSALDALDQ GLDALEEFRV EYFGKKGHFT ALMQELRNVA AEERPAIGAK 70 80 90 100 110 120 INEAKQTILD ILNAKKEAWE QEALNAKLAA ESIDVSLPGR KTELGGLHPV SITIERVVKF 130 140 150 160 170 180 FSELGFTVAS GPEIETDYYN FDALNIPAHH PARADHDTFW FDAQRLLRTQ TSGVQIRTME 190 200 210 220 230 240 NMQPPIRIVA PGRVYRNDYD QTHTPMFHQI ELLYVDKKAN FTELKGLIHD FLKAFFEEDL 250 260 270 280 290 300 QVRFRPSFFP FTEPSAEVDV MRQNGKWLEV LGCGMVHPNV LRNVGIDPEE YSGFAVGMGV 310 320 ERLTMLRYNV TDLRSFFEND LRFLKQFK

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61306.1.
RefSeq	YP_001968448.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3H137.
ModBase	Search...
Protein-protein interaction databases
STRING	B3H137.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6396400.
GenomeReviews	Gene locus APP7_0654 in contig CP001091_GR.
KEGG	apa:APP7_0654.
PATRIC	20754923. VBIActPle116979_0673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ACTP7

Accession

Primary (citable) accession number: B3H137

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program