Reviewed, UniProtKB/Swiss-Prot B3H120 (GUAA_ACTP7)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	APP7_0637

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120200
Regions
	Domain	8 – 205	198	Glutamine amidotransferase type-1
	Domain	237 – 399	163	GMP-binding
	Nucleotide binding	233 – 239	7	ATP By similarity
Sites
	Active site	85	1	Nucleophile By similarity
	Active site	179	1	By similarity
	Active site	181	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H120-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 40AD70FB471DDDD7	FASTA	523	58,197
	10 20 30 40 50 60 MTNIHNHKIL ILDFGSQYTQ LIARRVREIG VYCELWAWDV TEEQIREFNP TGIILSGGPE 70 80 90 100 110 120 STTEENSPRA QEYVFNAGVP VLGICYGMQT MAMQLGGLTE TSDHREFGYA SVDLQATDAL 130 140 150 160 170 180 FAKLNDNLTA SEPKLDVWMS HGDKVTRLAQ GFQVTGITPT CPIAAMSDES RRFYGVQFHP 190 200 210 220 230 240 EVTHTKSGLE LLTNFVVGIC GCECKWTAEN IIEDAVARIK EQVGDDEVIL GLSGGVDSSV 250 260 270 280 290 300 TALLLHRAIG KNLHCVFVDN GLLRLNEGDQ VMEMFGDKFG LNIIRVNAED RFLDALKGID 310 320 330 340 350 360 EPEAKRKTIG KVFVDVFDDE SKKLTSVKWL AQGTIYPDVI ESAASKTGKA HVIKSHHNVG 370 380 390 400 410 420 GLPDYMKLGL VEPLRELFKD EVRKIGLALG LPAEMLNRHP FPGPGLGVRV LGEIKKEYCD 430 440 450 460 470 480 LLRKADAIFI EELYKADWYY KVSQAFTVFL PVKSVGVMGD GRKYDWVVSL RAVETIDFMT 490 500 510 520 AHWAHLPYDL LGKISNRIIN EVNGISRVVY DVSGKPPATI EWE

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61289.1.
RefSeq	YP_001968431.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396394.
GenomeReviews	Gene locus APP7_0637 in contig CP001091_GR.
KEGG	apa:APP7_0637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	MKLVEPL.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ACTP7

Accession

Primary (citable) accession number: B3H120

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)