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B3H120 (GUAA_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing]

EC=6.3.5.2
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene names
Name:guaA
Ordered Locus Names:APP7_0637
OrganismActinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]
Taxonomic identifier537457 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the synthesis of GMP from XMP By similarity. HAMAP-Rule MF_00344

Catalytic activity

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP-Rule MF_00344

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP-Rule MF_00344

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00344

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523GMP synthase [glutamine-hydrolyzing] HAMAP-Rule MF_00344
PRO_1000120200

Regions

Domain8 – 205198Glutamine amidotransferase type-1
Domain206 – 398193GMPS ATP-PPase
Nucleotide binding233 – 2397ATP By similarity

Sites

Active site851Nucleophile By similarity
Active site1791 By similarity
Active site1811 By similarity

Sequences

Sequence LengthMass (Da)Tools
B3H120 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 40AD70FB471DDDD7

FASTA52358,197
        10         20         30         40         50         60 
MTNIHNHKIL ILDFGSQYTQ LIARRVREIG VYCELWAWDV TEEQIREFNP TGIILSGGPE 

        70         80         90        100        110        120 
STTEENSPRA QEYVFNAGVP VLGICYGMQT MAMQLGGLTE TSDHREFGYA SVDLQATDAL 

       130        140        150        160        170        180 
FAKLNDNLTA SEPKLDVWMS HGDKVTRLAQ GFQVTGITPT CPIAAMSDES RRFYGVQFHP 

       190        200        210        220        230        240 
EVTHTKSGLE LLTNFVVGIC GCECKWTAEN IIEDAVARIK EQVGDDEVIL GLSGGVDSSV 

       250        260        270        280        290        300 
TALLLHRAIG KNLHCVFVDN GLLRLNEGDQ VMEMFGDKFG LNIIRVNAED RFLDALKGID 

       310        320        330        340        350        360 
EPEAKRKTIG KVFVDVFDDE SKKLTSVKWL AQGTIYPDVI ESAASKTGKA HVIKSHHNVG 

       370        380        390        400        410        420 
GLPDYMKLGL VEPLRELFKD EVRKIGLALG LPAEMLNRHP FPGPGLGVRV LGEIKKEYCD 

       430        440        450        460        470        480 
LLRKADAIFI EELYKADWYY KVSQAFTVFL PVKSVGVMGD GRKYDWVVSL RAVETIDFMT 

       490        500        510        520 
AHWAHLPYDL LGKISNRIIN EVNGISRVVY DVSGKPPATI EWE 

« Hide

References

[1]"Genome and proteome analysis of A. pleuropneumoniae serotype 7."
Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AP76.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001091 Genomic DNA. Translation: ACE61289.1.
RefSeqYP_001968431.1. NC_010939.1.

3D structure databases

ProteinModelPortalB3H120.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING537457.APP7_0637.

Protein family/group databases

MEROPSC26.957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE61289; ACE61289; APP7_0637.
GeneID6396394.
KEGGapa:APP7_0637.
PATRIC20754891. VBIActPle116979_0657.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0519.
HOGENOMHOG000223964.
KOK01951.
OMAEIKKEYC.
OrthoDBEOG6JHRJV.
ProtClustDBPRK00074.

Enzyme and pathway databases

BioCycAPLE537457:GJI0-650-MONOMER.
UniPathwayUPA00189; UER00296.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00344. GMP_synthase.
InterProIPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUAA_ACTP7
AccessionPrimary (citable) accession number: B3H120
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways