Reviewed, UniProtKB/Swiss-Prot B3H0S9 (GLO2_ACTP7)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	APP7_0439

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144748
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	109	1	Zinc 1 By similarity
	Metal binding	127	1	Zinc 1 By similarity
	Metal binding	127	1	Zinc 2 By similarity
	Metal binding	165	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H0S9-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: F7510B0068980364	FASTA	235	26,642
	10 20 30 40 50 60 MLNITPIPAL SDNYIWAIQK DNDVIIVDPS DAVPVLAFIA KNQLNLTAIL LTHNHHDHTD 70 80 90 100 110 120 GMPELLSRYP QLSVYGPQEV AQFANRIVQP EDHLTLFDYD VRVIESAGHT AQHVSYLFGN 130 140 150 160 170 180 EYLFCGDVLF SGGCGRVFTG NYQAQFDALQ RFKALPEFVE IFPAHEYTLS NLKFAEAVLA 190 200 210 220 230 PSCALFEIQE RAEILRSRNQ PTLPTTLERE LQINPFLQAV DLDQFIALRH QKDNF

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61091.1.
RefSeq	YP_001968233.1.
3D structure databases
SMR	B3H0S9. Positions 2-235.
ModBase	Search...
Genome annotation databases
GeneID	6396778.
GenomeReviews	Gene locus APP7_0439 in contig CP001091_GR.
KEGG	apa:APP7_0439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	WCAHEYT.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_ACTP7

Accession

Primary (citable) accession number: B3H0S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)