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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:APP7_0431
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001226 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001226811 – 264Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST264

Proteomic databases

PRIDEiB3H0S1.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB3H0S1.
SMRiB3H0S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000294326.
KOiK00677.
OMAiVQDRSET.
OrthoDBiPOG090M00XJ.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiView protein in InterPro
IPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
PfamiView protein in Pfam
PF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiView protein in PROSITE
PS00101. HEXAPEP_TRANSFERASES. 1 hit.

Sequencei

Sequence statusi: Complete.

B3H0S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLIDSTAKI SPLAVVEEGA QIGAHVEIGP FSVIGKNVKI GAKTIIHSHV
60 70 80 90 100
VINGHTEIGE QNQIFQFASI GEINQDLKYQ GEPTKVIIGN RNRIRESVTI
110 120 130 140 150
HRGTVQGGGV TRIGNDNLFM INTHIAHDCS IGNRCIIANN GTLAGHVTLD
160 170 180 190 200
DFVIVGGMSA IHQFVVIGSH VMLGGGSMVS QDVPPYVMAQ GNHAQPFGVN
210 220 230 240 250
LEGLKRRGFD KPAMHAIRNA YKLIYRSGKT IEEAIPEIEQ FAVNEPAVQL
260
FLDFFKRSTR GIIR
Length:264
Mass (Da):28,721
Last modified:July 22, 2008 - v1
Checksum:i46C75D4AFFD97AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE61083.1.
RefSeqiWP_005616848.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE61083; ACE61083; APP7_0431.
KEGGiapa:APP7_0431.
PATRICi20754455. VBIActPle116979_0442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA. Translation: ACE61083.1.
RefSeqiWP_005616848.1. NC_010939.1.

3D structure databases

ProteinModelPortaliB3H0S1.
SMRiB3H0S1.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB3H0S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE61083; ACE61083; APP7_0431.
KEGGiapa:APP7_0431.
PATRICi20754455. VBIActPle116979_0442.

Phylogenomic databases

HOGENOMiHOG000294326.
KOiK00677.
OMAiVQDRSET.
OrthoDBiPOG090M00XJ.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiView protein in InterPro
IPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
PfamiView protein in Pfam
PF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiView protein in PROSITE
PS00101. HEXAPEP_TRANSFERASES. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXA_ACTP7
AccessioniPrimary (citable) accession number: B3H0S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: February 15, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.