Reviewed, UniProtKB/Swiss-Prot B3H0J6 (NDK_ACTP7)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase
      Short name=NDK
      Short name=NDP kinase
    EC=2.7.4.6
Alternative name(s):
    Nucleoside-2-P kinase

Gene names

Name:	ndk
Ordered Locus Names:	APP7_0356

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP MF_00451
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: HAMAP GTP biosynthetic process Inferred from electronic annotation. Source: HAMAP UTP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Nucleoside diphosphate kinase HAMAP MF_00451		PRO_1000192251
Sites
	Active site	116	1	Pros-phosphohistidine intermediate By similarity
	Binding site	10	1	ATP By similarity
	Binding site	58	1	ATP By similarity
	Binding site	86	1	ATP By similarity
	Binding site	92	1	ATP By similarity
	Binding site	103	1	ATP By similarity
	Binding site	113	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H0J6-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 9859E18A88626D30	FASTA	138	15,359
	10 20 30 40 50 60 MIQQTLCLIK PDATQRNLIG KILSHLEEAG LTIKALKKVQ LNQEQAEGFY AEHQGKEFFA 70 80 90 100 110 120 PLVEFMISAP IVAVVLEGEN AISHYRELMG ATNPEQRKAG TIRALYAISG RENSVHGSDS 130 EQSAKREIAY FFTPNEIL

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61008.1.
RefSeq	YP_001968150.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396931.
GenomeReviews	Gene locus APP7_0356 in contig CP001091_GR.
KEGG	apa:APP7_0356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NLTGAIT.
Family and domain databases
HAMAP	MF_00451. [Tree]
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK_ACTP7

Accession

Primary (citable) accession number: B3H0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)