Reviewed, UniProtKB/Swiss-Prot B3H0J1 (GLGB_ACTP7)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	APP7_0351

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000131805
Sites
	Active site	303	1	By similarity
	Active site	338	1	By similarity
	Active site	343	1	By similarity
	Active site	406	1	By similarity
	Active site	408	1	By similarity
	Active site	461	1	By similarity
	Active site	528	1	By similarity
	Active site	529	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H0J1-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 721D6F0872F6BA8B	FASTA	777	89,290
	10 20 30 40 50 60 MKTYTYSKQD LSFIEQLSQA YCKDPFSYLG LHQAGDVSVI RVFLPEATTV KILSADGQIL 70 80 90 100 110 120 SEALKIDDSG LFVAQLSQQY SSLNYRLRVG YSLAEIDLED PYRFTSSLLP MDNWLLAEGT 130 140 150 160 170 180 HLRPYEILGA HLKTQEGVSG VHFSVWAPNA RRVSVVGDFN YWDGRVNPMR FHAESGIWDI 190 200 210 220 230 240 FLPNVEKGAL YKFEILDSNG NIRLKSDPYA FASQFRPDTA SVVTGLPEKI EVDAKLRHAN 250 260 270 280 290 300 DPDQPISIYE VHLGSWRRHL ENNYWLNYEE IANELIPYVK DMGFTHIELL PITEYPFDGS 310 320 330 340 350 360 WGYQPTGLYS PTSRFGSPDD LRTLIRKAHE AGINVILDWV VGHFPTDSHG LTEFDGSHLY 370 380 390 400 410 420 EHQDPREGYH QDWNTLIFNY GRHEVFNYLS SNALYWTERF GIDGLRVDAV SSMIYRDYSR 430 440 450 460 470 480 KDGEWIPNQY GGRENLEALD FLRRTNRMLK KEGHGAVVIA EESTSFAGIT HSPTENGVGF 490 500 510 520 530 540 DYKWNMGWMN DTLRYMSLDP IYRQYHHDWM TFGMMYQYSE KFVLPLSHDE VVHGKCSILG 550 560 570 580 590 600 KMSGDCWQKF ANLRAYYGYM WGYPGKKLLF MGNEFAQGRE WNYNESLDWF LLGEQGGGWH 610 620 630 640 650 660 KGVLNWVRDL NRTYQKYPAL YQLDYDPAGF EWLVVDDWQQ SVFAFERKAK NGESVIVVSN 670 680 690 700 710 720 FTPVVRHNYR IGVRQDGTYT EILNSDAAYY EGSNVGNYGE IECEAIESHG KPFSIELSIP 730 740 750 760 770 PLSTIFIACQ PKPKEAVEAE QDIVKMAEVA MQKALKPTKK TVSVKAKAHK KAHKNKK

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61003.1.
RefSeq	YP_001968145.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396940.
GenomeReviews	Gene locus APP7_0351 in contig CP001091_GR.
KEGG	apa:APP7_0351.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HERCPHA.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ACTP7

Accession

Primary (citable) accession number: B3H0J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)