ID   B3H0I4_ACTP7            Unreviewed;       879 AA.
AC   B3H0I4;
DT   22-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   22-JUL-2008, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=pepC {ECO:0000313|EMBL:ACE60996.1};
GN   Synonyms=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN   OrderedLocusNames=APP7_0344 {ECO:0000313|EMBL:ACE60996.1};
OS   Actinobacillus pleuropneumoniae serotype 7 (strain AP76).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=537457 {ECO:0000313|EMBL:ACE60996.1, ECO:0000313|Proteomes:UP000001226};
RN   [1] {ECO:0000313|Proteomes:UP000001226}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AP76 {ECO:0000313|Proteomes:UP000001226};
RA   Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N.,
RA   Tegetmeyer H., Singh M., Gerlach G.F.;
RT   "Genome and proteome analysis of A. pleuropneumoniae serotype 7.";
RL   Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP001091; ACE60996.1; -; Genomic_DNA.
DR   RefSeq; WP_005619892.1; NC_010939.1.
DR   AlphaFoldDB; B3H0I4; -.
DR   KEGG; apa:APP7_0344; -.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   Proteomes; UP000001226; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:ACE60996.1}.
FT   ACT_SITE        138
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        545
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   879 AA;  99170 MW;  B40E41DCC8AC2C4C CRC64;
     MNQPYSAMRN NIHMLGDFLG ETIREAQGNE ILELIESIRV LSRDSRAGDE KAREQLLDKL
     ANISTENVLP VARAFSQFLN LTNIAEQYQT ISRHHQDESL GNRSLSALFA RLKAQGTPVE
     TVIKTVEKLL IELVLTAHPT EVTRRSLVHK HVEINKCLGQ LEHDDLTEAE QTKLKRRLMQ
     LIALAWHTNE IRAARPTPVD EAKWGMAIIE NSLWKAVPDF CRELNFQLEK HFDVQYEVAL
     SPVRFSSWMG GDRDGNPFVT AEITQKVLRM NRWKAAELFL NDVSDLVEEL SIVQCTPEFR
     EKYGDHIEPY RVVVKGLRSK LQKTLTYFGE LIEAKPTTVD PSEIITCDND LWEPLYDCYQ
     SLHACGMRII ANGSLLDCLR RIRCFGLGLS RLDIRQESSR HETAIAEITR YIGLGDYAQW
     TEDDKQAFLV RELSSRRPLV PTNWTPSPET QEILDTCKVV AQQPEGTISA YVISMAREAS
     DVLAVHLLLK EAGCNYTLPV APLFETLDDL DHSEKVMTDL FNIGWYRGVI NNYQMVMIGY
     SDSAKDAGML AASWAQYRAQ EALVNLAEKY HIELVLFHGR GGTVGRGGAP AHAALLSQPP
     RSLKSGLRVT EQGEMIRFKL GLPAVAVETL NLYASAILEA NLLPPPEPKQ KWRDIMDKGA
     AISCEIYRGV VRGEPDFVPY FRSATPEQEL GKLPLGSRPS KRNPNGGVES LRAIPWIFAW
     MQNRLMLPAW LGAGAALRQM IEQGEESTLK EMCNEWPFFS TRIGMLEMVF SKADLWLAEH
     YDQRLVAKEL HRLGKTLRAQ LSADIQTVLT LAHDGQLMAD LPWIAESIAL RNVYTDPLNL
     LQVELLQRLR SQGEVRDPQL EQALMITITG IAAGMRNTG
//