Skip Header

Contribute Send feedback
Read comments (?) or add your own

B3H0G3 (PYRE_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:APP7_0323
OrganismActinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]
Taxonomic identifier537457 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_1000138757

Regions

Region34 – 352Orotate binding By similarity
Region72 – 7325-phosphoribose 1-diphosphate binding By similarity
Region124 – 13295-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site2615-phosphoribose 1-diphosphate By similarity
Binding site9915-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10015-phosphoribose 1-diphosphate By similarity
Binding site10315-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10515-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1281Orotate By similarity
Binding site1561Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
B3H0G3 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 3F89FE5BCC66BA26

FASTA21323,684
        10         20         30         40         50         60 
MEQYKHDFIE FALSRNVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLAKLG EFYAQAIQAS 

        70         80         90        100        110        120 
GLNFDVLFGP AYKGIPIATT VAVALVNQFD VDKPCCFNRK EAKDHGEGGN LIGSPLKGRI 

       130        140        150        160        170        180 
LLVDDVITAG TAIRESMEII NANQAELAGV LIALNRKEKG KGELSAIQEV ERDYGCQVFS 

       190        200        210 
IIDFDDLIQF IEKSEQYAPY LASMRAYREQ YGV

« Hide

References

[1]"Genome and proteome analysis of A. pleuropneumoniae serotype 7."
Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AP76.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001091 Genomic DNA. Translation: ACE60975.1.
RefSeqYP_001968117.1. NC_010939.1.

3D structure databases

ProteinModelPortalB3H0G3.
SMRB3H0G3. Positions 1-213.
ModBaseSearch...

Protein-protein interaction databases

STRINGB3H0G3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6396995.
GenomeReviewsGene locus APP7_0323 in contig CP001091_GR.
KEGGapa:APP7_0323.
PATRIC20754233. VBIActPle116979_0331.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG404341.
OMAFAFNRKE.
ProtClustDBPRK00455.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_ACTP7
AccessionPrimary (citable) accession number: B3H0G3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents