Reviewed, UniProtKB/Swiss-Prot B3H061 (SYT_ACTP7)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	APP7_0221

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000098537
Regions
	Region	243 – 534	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	511	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3H061-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 6A3EF0126EC3D771	FASTA	643	73,533
	10 20 30 40 50 60 MPIITLPDGS QRQFDNPVSV MEVAQSIGAG LAKATIAGRV NGERRDACDI ISEDSSLEII 70 80 90 100 110 120 TAKDEDGLEI IRHSCAHLLG HAIKQLFPDV KMAIGPTIDN GFYYDVDLDR SLTQEDLDAI 130 140 150 160 170 180 EKRMLELAKT NYDVVKKRVS WQEARDTFEK RGEPYKMAIL DENIERTATP ALYHHEEYID 190 200 210 220 230 240 MCRGPHVPNM RFCHHFKLQK VAGAYWRGDS KNKMLQRIYG TAWADKKQLA EYLTRLEEAA 250 260 270 280 290 300 KRDHRRIGKA LDLYHMQEEA PGLVFWHNDG WTIFRELETF VRTKLKEYDY QEVKGPFMMD 310 320 330 340 350 360 RVLWERTGHW QNYADLMFTT QSENREYAIK PMNCPGHVQI FNQGLKSYRD LPIRMAEFGS 370 380 390 400 410 420 CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE DQIESEVTSC IRMVYDIYST FGFSNIQVKL 430 440 450 460 470 480 STRPENRIGD DAMWDRAEDG LAKALTANGL SYEIQEGEGA FYGPKIEFAL RDCLDREWQC 490 500 510 520 530 540 GTIQLDFALP GRLDASYVAE DNGRRTPVMI HRAILGSIER FIGIITEEYA GFFPTWLAPT 550 560 570 580 590 600 QAVVMNITDS QADYVQKVTK ALSDAGIRAK SDLRNEKVGF KVREHTLRRV PYMLVCGDKE 610 620 630 640 IEAGKVSVRT RKGADLGTFT IDEFVEILKN QVKARGLKLL GEE

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE60873.1.
RefSeq	YP_001968015.1.
3D structure databases
SMR	B3H061. Positions 2-642.
ModBase	Search...
Genome annotation databases
GeneID	6397193.
GenomeReviews	Gene locus APP7_0221 in contig CP001091_GR.
KEGG	apa:APP7_0221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	FCQHFTL.
Family and domain databases
HAMAP	MF_00184_B. Thr_tRNA_synth_B. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACTP7

Accession

Primary (citable) accession number: B3H061

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)