B3H050 (DXS_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	APP7_0210

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115715

Sequences

	Sequence		Length	Mass (Da)
	B3H050 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 5573F10785EE1476	FASTA	617	67,842
	10 20 30 40 50 60 MQNKYPLLSQ INSPEDLRLL AKEQLQSVAD ELRAYLLESV SQTSGHLASG LGVVELTVAL 70 80 90 100 110 120 HYVYQTPFDQ LIWDVGHQAY PHKILTGRRD QMHTIRQKNG IHPFPWREES LYDVLSVGHS 130 140 150 160 170 180 STSISAGVGI AVAAEKENAG RKTVCVIGDG AITAGMAFEA MNHAGALHTD MLVILNDNEM 190 200 210 220 230 240 SISENVGALN NHLARIFSGS IYTTVRDGSK KVLDKVPTIK NFMKKSEEHM KGVISPESTL 250 260 270 280 290 300 FEELGFNYIG PIDGHNIDEL VKTLSNMREL KGPQFLHIRT KKGKGYEPAE NDPIGYHGVP 310 320 330 340 350 360 KFDPTCGQLP KSKTIPTYSD IFGNWLCEMA EQDSKLIGIT PAMREGSGMV EFSKRFPEQY 370 380 390 400 410 420 FDVAIAEQHA VTFGAGLAIA GYKPVVAIYS SFLQRAYDQL IHDVAIQNLP VIFAIDRAGI 430 440 450 460 470 480 VGADGQTHQG AFDVSFMRCI PNMTIMCPSD ENEMRQMLYT AYRMNTPTAV RYPRGNAQGV 490 500 510 520 530 540 ALAPMQALEV GKGKLIQQGQ KVAILNFGPL LNEARIVAEK HNYTLADMRF VKPLDEQLVA 550 560 570 580 590 600 ELADSHELLV TLEENAIQGG AGSAVNEYLQ KIGKIRPLVM LGIPDFFVPQ ATQAESYADL 610 GLDAAGIEQR IQAVVNQ

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE60862.1.
RefSeq	YP_001968004.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3H050.
SMR	B3H050. Positions 4-613.
ModBase	Search...
Protein-protein interaction databases
STRING	B3H050.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6397217.
GenomeReviews	Gene locus APP7_0210 in contig CP001091_GR.
KEGG	apa:APP7_0210.
PATRIC	20753981. VBIActPle116979_0212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ACTP7

Accession

Primary (citable) accession number: B3H050

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program