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B3GZV8

- BIOB_ACTP7

UniProt

B3GZV8 - BIOB_ACTP7

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Protein
Biotin synthase
Gene
bioB, APP7_0118
Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi113 – 1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi144 – 1441Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi204 – 2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi276 – 2761Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAPLE537457:GJI0-123-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:APP7_0118
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001226: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Biotin synthaseUniRule annotation
PRO_0000381181Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi537457.APP7_0118.

Structurei

3D structure databases

ProteinModelPortaliB3GZV8.
SMRiB3GZV8. Positions 23-328.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B3GZV8-1 [UniParc]FASTAAdd to Basket

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MTTYNLRLKA KSELTPHPTA QYWRKCQVEE LFEMPFMELI FKAAQVHREH    50
FDPQAIQLST LLSIKTGGCP EECEYCPQSA RYDTGLEKQV MMDVEEIVEK 100
AKIAKARGAS RFCMGAAWRG PKPKDIKVVS EIIGVVKALG LETCGTFGLL 150
EDGMAEDLKQ AGLDYYNHNL DTDPERYNKI VHTRNHDDRM DTLGKVRHAG 200
LKVCCGGIVG MNETRPERAG LIASLANLDP QPESVPINQL IKVEGTPLEN 250
AEDLDWTEFV RTIAVARITM PKSYVRLSAG RTSMPEAMQT LCFMAGANSI 300
FYGEKLLTTC NPEEDSDRLL MDKLDLYPLA YEPSDA 336
Length:336
Mass (Da):37,629
Last modified:July 22, 2008 - v1
Checksum:iE3CB246F62B10D9F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE60770.1.
RefSeqiYP_001967912.1. NC_010939.1.

Genome annotation databases

EnsemblBacteriaiACE60770; ACE60770; APP7_0118.
GeneIDi6397236.
KEGGiapa:APP7_0118.
PATRICi20753796. VBIActPle116979_0120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001091 Genomic DNA. Translation: ACE60770.1 .
RefSeqi YP_001967912.1. NC_010939.1.

3D structure databases

ProteinModelPortali B3GZV8.
SMRi B3GZV8. Positions 23-328.
ModBasei Search...

Protein-protein interaction databases

STRINGi 537457.APP7_0118.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE60770 ; ACE60770 ; APP7_0118 .
GeneIDi 6397236.
KEGGi apa:APP7_0118.
PATRICi 20753796. VBIActPle116979_0120.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci APLE537457:GJI0-123-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome analysis of A. pleuropneumoniae serotype 7."
    Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AP76.

Entry informationi

Entry nameiBIOB_ACTP7
AccessioniPrimary (citable) accession number: B3GZV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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