Reviewed, UniProtKB/Swiss-Prot B3GZK9 (MURC_ACTP7)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	APP7_0019

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000091073
Regions
	Nucleotide binding	125 – 131	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3GZK9-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 5DE7CC2681059DFC	FASTA	475	51,520
	10 20 30 40 50 60 MKNFQDKIKK LVPEMRRVNQ IHFIGIGGAG MSGIAEVLLN EGYQISGSDI AEGPVTKRLA 70 80 90 100 110 120 EAGAKVFIGH QAENVAGASV VVASSAIDDS NPEVRAAKEA RIPVIQRAQM LAEIMRFRHG 130 140 150 160 170 180 IAVAGTHGKT TTTAMISMIY TEAKLDPTFV NGGLVKSAGK NAHLGASRYL IAEADESDAS 190 200 210 220 230 240 FLHLQPMVSV VTNIEPDHMD TYGGDFEQMK ATYVKFLRNL PFYGLAVMCA DDETVMEIAP 250 260 270 280 290 300 QVGRQVLTYG FSEKADYRIE DYQQTGFQGH YTVVCPNGER IDVLLNVPGK HNALNATAAL 310 320 330 340 350 360 AVAKEEGIAN EAILAALADF QGAGRRFDQL GSFIRPNGKV MLVDDYGHHP TEVDVTIKAA 370 380 390 400 410 420 RSGWENKRVV MIFQPHRYSR TRDLFDDFVQ VLSQVDALIM LEVYAAGEAP IVGADSKALC 430 440 450 460 470 RSIRNLGKVD PILVSDTDQL GEVLDQIIQD GDLILAQGAG SVSRISRGLA ESWKA

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE60671.1.
RefSeq	YP_001967813.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6397597.
GenomeReviews	Gene locus APP7_0019 in contig CP001091_GR.
KEGG	apa:APP7_0019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACTP7

Accession

Primary (citable) accession number: B3GZK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)