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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciAPLE537457:G1GC5-7-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:APP7_0007
OrganismiActinobacillus pleuropneumoniae serotype 7 (strain AP76)
Taxonomic identifieri537457 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001226 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001914571 – 393Lipid-A-disaccharide synthaseAdd BLAST393

Structurei

3D structure databases

ProteinModelPortaliB3GZJ7
SMRiB3GZJ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

B3GZJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKEAPLIA LVAGEISGDI LGAGLINALK LHYPNARFIG VAGPRMIQAG
60 70 80 90 100
CETLFDMEEL AVMGLAEVVK HLPRLLKRRK QVIETMLAEK PDIFIGIDAP
110 120 130 140 150
DFNLTVEEKL KASGIKTIHY VSPSVWAWRQ NRVQKIARAT NLVLAFLPFE
160 170 180 190 200
KAFYDRFNVP CRFIGHTMAD AIALKPNRSE ACATLNLDET QRYLAILVGS
210 220 230 240 250
RASEVRFLAE PFLKAAKILK QQYPDLQFLV PLVNDKRIAQ FEQIKAQVAP
260 270 280 290 300
ELSVHILKGN ARQAMIAAEA SLLASGTAAL EGMLCKSPMV VGYKMKAMTY
310 320 330 340 350
WLAKRLVKTK YISLPNLLAD EMLVPELIQD ECNPENLAWY LGNYLADDAD
360 370 380 390
HRKQRNELKQ RFTELHKLIQ CDADAQAAQA VVDVLEANTS DQN
Length:393
Mass (Da):43,880
Last modified:July 22, 2008 - v1
Checksum:iEAA9F7279B6442ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001091 Genomic DNA Translation: ACE60659.1
RefSeqiWP_012478271.1, NC_010939.1

Genome annotation databases

EnsemblBacteriaiACE60659; ACE60659; APP7_0007
KEGGiapa:APP7_0007

Similar proteinsi

Entry informationi

Entry nameiLPXB_ACTP7
AccessioniPrimary (citable) accession number: B3GZJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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