Reviewed, UniProtKB/Swiss-Prot B3GZ95 (CYSI_ACTP7)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	APP7_1929

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000146639
Sites
	Metal binding	442	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	448	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	487	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron (siroheme axial ligand) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GZ95-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: F9ECBDA137F696FD	FASTA	588	66,663
	10 20 30 40 50 60 MSDKKTKGLE WQEKPLSDNE RLKTDSNFLR GTILDDLKDG LTGGFKGDNF QLIRFHGMYE 70 80 90 100 110 120 QDDRDIRAER LEEKLEPLKF MLLRCRLPGG IIKPYQWIEI DKFAREHTRY QSIRLTNRQT 130 140 150 160 170 180 FQYHGVPKGK LQPMHRLLHS IGLDSIATAA DMNRNVLCTS NPIESELHQQ AYEFAKKISE 190 200 210 220 230 240 HLLPRSRGYL DVWVDGKKVE SSDDLLKIED EPILGKTYLP RKFKTAVAIP PLNDVDVYAN 250 260 270 280 290 300 DLNFIAIQDE NGQLCGFNVL VGGGLSFEHG NTKTYPNVAY SLGFVPLEHT LAAAEGVVKT 310 320 330 340 350 360 QRDFGNRSDR KNARVRYTIQ NMTLDGFRAE VERCMNIKFE PTRPYEFTER GDRIGWVKGI 370 380 390 400 410 420 DNNWHLTLFI ESGRITDKPE KPLMTGVLEL AKVHKGDFRI TANQNLIVAN VAEQDKAQIE 430 440 450 460 470 480 AIARQYGLIQ EISKLRENAM SCVSLPTCPL AMAEAERVLP DFISELDKVL SKHNVADESI 490 500 510 520 530 540 ITRITGCPNG CGRAMLAEIG LVGKAIGRYN LHIGGDRAGL RISRLYKENI TLQEIVNEID 550 560 570 580 QLVARWATER QTNEAFGDFV IRSNIIAPVV NAHIDFWDAT KIIPTTII

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE62581.1.
RefSeq	YP_001969723.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6397824.
GenomeReviews	Gene locus APP7_1929 in contig CP001091_GR.
KEGG	apa:APP7_1929.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ITTTQWQ.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ACTP7

Accession

Primary (citable) accession number: B3GZ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)