Reviewed, UniProtKB/Swiss-Prot B3GZ82 (SYP_ACTP7)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	APP7_1916

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_1000199347

Sequences

	Sequence		Length	Mass (Da)
	B3GZ82-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: C96388A1E9A16F17	FASTA	571	63,574
	10 20 30 40 50 60 MRTSQYLFST LKETPNDAQV VSHQLMLRAG MIRPMASGLY NWLPTGIKVL KKVENIIREE 70 80 90 100 110 120 MNKGGAIEVL MPVVQPAELW QESGRWNDYG AELLRFVDRG SRDFVLGPTH EEVITDLVRR 130 140 150 160 170 180 EVSSYKQLPL NLYQIQTKFR DEVRPRFGVM RSREFVMKDA YSFHVDKASL QETYDVMYQV 190 200 210 220 230 240 YSNIFTRLGL DFRAVQADTG SIGGSASHEF QVLASSGEDD VVFSTESDFA ANIELAEAVA 250 260 270 280 290 300 VGERQAPTAE MQLVDTPNAK TINELVEQFN LPIEKTVKTL IVKGATEEQS LVALVLRGDH 310 320 330 340 350 360 ELNEIKAQKH PLVADPLEFA DEAEIKAKIG AGVGSLGVIN LNVPAIIDRS VAVMSDFGCG 370 380 390 400 410 420 ANIDGRHYFN VNWERDVAMP EVADLRNVVE GDPSPDGKGV LQIKRGIEVG HIFQLGTKYS 430 440 450 460 470 480 EAMKATVQGD DGKPLVMTMG CYGIGVTRVV AAAIEQHHDE RGIIWPSDEI APFTVAIVPM 490 500 510 520 530 540 NMHKSESVQQ FSEELYRTLK AQGVDVIFDD RKERPGVMFA DMELIGVPHM VVIGEKNLAN 550 560 570 GEIEYKNRRT GEKQMIAKDQ LLAFLKENVK A

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE62568.1.
RefSeq	YP_001969710.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6395982.
GenomeReviews	Gene locus APP7_1916 in contig CP001091_GR.
KEGG	apa:APP7_1916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACTP7

Accession

Primary (citable) accession number: B3GZ82

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)