B3GZ40 (TRMD_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	APP7_1874

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130124
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	117	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GZ40 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: F8DF5AE74E598724	FASTA	251	28,221
	10 20 30 40 50 60 MWIGIISLFP EMFKAITDFG VTGRAVKQDL LQIQCWNPRD FTFDKHKTVD DRPYGGGPGM 70 80 90 100 110 120 LMMVQPLRDA IHAAKQAAKA EDGVEAKVIY LSPQGRKLDQ QGVKTLASNQ KLILICGRYE 130 140 150 160 170 180 GVDERLIQTE VDEEWSIGDY VLTGGELPAM TLIDAVARFV PGVLGKQASA DEDSFATGLL 190 200 210 220 230 240 DCPHYTRPEM LDGIPVPEVL MSGHHENIRK WRLEQSLERT WLRRPELLDS LALTDEQRVL 250 LAKIKKQHKI S

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE62526.1.
RefSeq	YP_001969668.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3GZ40.
SMR	B3GZ40. Positions 1-248.
ModBase	Search...
Protein-protein interaction databases
STRING	B3GZ40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6397990.
GenomeReviews	Gene locus APP7_1874 in contig CP001091_GR.
KEGG	apa:APP7_1874.
PATRIC	20757537. VBIActPle116979_1919.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACTP7

Accession

Primary (citable) accession number: B3GZ40

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program