Reviewed, UniProtKB/Swiss-Prot B3GYT7 (DEF_ACTP7)

Names and origin

Protein names

Recommended name:
    Peptide deformylase
      Short name=PDF
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase

Gene names

Name:	def
Ordered Locus Names:	APP7_1771

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Peptide deformylase HAMAP MF_00163		PRO_1000097292
Sites
	Active site	134	1	By similarity
	Metal binding	91	1	Iron By similarity
	Metal binding	133	1	Iron By similarity
	Metal binding	137	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GYT7-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 8D223CD26AE35D32	FASTA	170	19,087
	10 20 30 40 50 60 MSVLEVVLYP DEGLAKVCEP VAQVDDELNQ FIDDMFDTMY EHEGIGLAAP QVGVLKRVIT 70 80 90 100 110 120 IDIEGDKTNQ VVLINPEILE SCGETGIEEG CLSIPGHRAL VPRKEKVKVK ALNRKGEEVI 130 140 150 160 170 YEADGLFAIC IQHEIDHLNG VLFVDHISAL KRQRIKEKMQ KLKKQIERAK

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE62423.1.
RefSeq	YP_001969565.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396576.
GenomeReviews	Gene locus APP7_1771 in contig CP001091_GR.
KEGG	apa:APP7_1771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CIQHECD.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF_ACTP7

Accession

Primary (citable) accession number: B3GYT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)