Reviewed, UniProtKB/Swiss-Prot B3GY43 (METE_ACTP7)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	APP7_1214

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097812
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GY43-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 5DAF04387ED0F356	FASTA	757	85,888
	10 20 30 40 50 60 MTTLHILGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWLHQ AQADADFVTV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQTL SLDQYFQLAR GNKEQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFQKNTT FKANPTHYVN QIREAKAAGH QVKPVIVGPL TFLWLGKEKG EAFNRFDLLK 190 200 210 220 230 240 QLVPIYFEIL TSLAAEGVEW IQIDEPALAL DLPQEWLAAY QDVYAQLAKV NAKLLLATYF 250 260 270 280 290 300 GSVAEHADLL KALPVDGLHL DLVRAPEQLS AFEDYPKVLS AGVIDGRNIW RANLNRVLDV 310 320 330 340 350 360 LEPLKAKFNQ RLWIAPSCSL LHTPYDLAVE TQLQQNNPEL YRWLAFTLQK VAELQVLKQA 370 380 390 400 410 420 LNAGRSAVAE QLNDSQTAAD ARANSNVIHK AEVAQRLANL PTNADKRQSP FAERIKLQNK 430 440 450 460 470 480 WLNLPLLPTT NIGSFPQTLE IRHARAAFKK GELSLADYEA AMKKEIEFVV RKQEELDLDV 490 500 510 520 530 540 LVHGEAERND MVEYFGELLD GFAFTKFGWV QSYGSRCVKP PVIYGDVTRP EPMTVRWSQY 550 560 570 580 590 600 AQSLTDKVMK GMLTGPVTIL QWSFVRNDIP RSTVCKQIGV ALSDEVLDLE KAGIKVIQID 610 620 630 640 650 660 EPAIREGLPL KRADWDAYLQ WAGEAFRLSY MGVKDDTQIH THMCYSEFND ILPAIAGLDA 670 680 690 700 710 720 DVITIETSRS DMELLTAFAD FKYPNDIGPG VYDIHSPRVP TAGEIEHLLR KALKVIPKER 730 740 750 LWVNPDCGLK TRGWKETIEQ LQVMVEVTKK LRAELAE

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61866.1.
RefSeq	YP_001969008.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6397409.
GenomeReviews	Gene locus APP7_1214 in contig CP001091_GR.
KEGG	apa:APP7_1214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ACTP7

Accession

Primary (citable) accession number: B3GY43

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)